Expression of bcl-2 and bax in regenerating rat renal tubules following ischemic injury

The American Journal of Physiology
David P BasileM R Hammerman

Abstract

To define potential roles for bcl-2 and bax in adult kidney as regulators of regeneration, their expressions were characterized postischemic injury. A 2.1-fold increase in levels of renal bcl-2 mRNA occurred within 24 h of injury relative to levels in kidney of sham-operated control rats. The levels of bcl-2 mRNA remained elevated for 3 days but returned to baseline by day 5 postischemia. In situ hybridization of kidneys from sham-operated rats demonstrated faint expression of bcl-2 mRNA localized diffusely throughout the nephron. After renal injury, the expression of bcl-2 mRNA was markedly enhanced in regenerating proximal tubule cells relining the basement membrane. Immunohistochemistry showed a similar localization for bcl-2 protein. Levels of bax mRNA in kidney were elevated beginning at 24 h postischemia and remained elevated for 7 days postinjury. Bax mRNA and bax protein were colocalized to regenerating proximal tubules postischemia and were prominently expressed in papillary proliferations. We conclude that the expressions of bcl-2 and bax in kidney are enhanced in a predictable pattern following acute ischemic injury. Our findings suggest that these regulators of apoptosis play key roles in the process of repair of th...Continue Reading

Citations

Mar 7, 2003·American Journal of Physiology. Renal Physiology·Babu J Padanilam
Aug 4, 2006·American Journal of Physiology. Renal Physiology·Craig BrooksZheng Dong
May 8, 2009·American Journal of Physiology. Renal Physiology·Dina A AyupovaBeth S Lee
Dec 18, 2003·American Journal of Physiology. Renal Physiology·Qingqing WeiZheng Dong
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Mar 10, 2005·American Journal of Physiology. Renal Physiology·Craig BrooksZheng Dong
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Apr 29, 2004·American Journal of Physiology. Renal Physiology·Qingqing WeiZheng Dong
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Dec 4, 2010·Journal of the American Society of Nephrology : JASN·Caitlin C O'MearaHoward J Jacob
Jun 18, 2021·Advanced Healthcare Materials·Rita Sobreiro-AlmeidaNuno M Neves
Feb 22, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Patricia Y W DankersMarja J A van Luyn

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