PMID: 9180925May 1, 1997Paper

Expression of bcl-2, bax, and bcl-XL proteins in azoxymethane-induced rat colonic adenocarcinomas

Molecular Carcinogenesis
Y HiroseH Mori

Abstract

Using western blotting and immunochemical analysis, we investigated alterations in the expression of the apoptosis-related proteins bcl-2, bax, and bcl-X in colonic adenocarcinomas induced by subcutaneous injection of azoxymethane (AOM) (15 mg/kg body weight weekly for 2 wk) into male Sprague-Dawley rats. Expression of the apoptosis-repressor bcl-2 in the colonic tumors was significantly weaker (0.6-fold) than that in adjacent non-neoplastic mucosa. The expression of bax protein, an apoptosis accelerator, was significantly stronger (7.33-fold) in all the tumors than in the non-tumoral mucosa. bcl-XL protein, which functions as a repressor of apoptosis, was significantly upregulated (3.23-fold) in all the tumors when compared with the non-neoplastic mucosa. There was no significant difference between the expression of these proteins in the non-neoplastic mucosa of the AOM-treated rats and in the normal mucosa of saline-treated control rats. As determined by immunohistochemical analysis, the tumor cells had more bax and bcl-X protein. These findings indicate that the regulation of the apoptosis-related proteins bcl-2, bax, and bcl-XL was altered in the AOM-induced colonic neoplastic tissue. In terms of resistance to apoptosis, el...Continue Reading

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Citations

Jul 25, 2009·Journal of Pharmaceutical Sciences·Chie TakayamaToshikiro Kimura
Apr 25, 2000·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·V N AnisimovI Zusman
Feb 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Agneta Jansson, Xiao-Feng Sun
Mar 29, 2000·Journal of Gastroenterology and Hepatology·A RamachandranK A Balasubramanian
May 2, 2001·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·R H DashwoodD T Horio

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