Expression of BCL10 in cervical cancer has a role in the regulation of cell growth through the activation of NF-κB-dependent cyclin D1 signaling

Gynecologic Oncology
Sung-Hsin KuoRuey-Jien Chen

Abstract

We recently characterized the molecular linkage that directs both BCL10 overexpression and nuclear translocation in response to inflammation-related NF-κB signaling pathway. Since NF-κB activation has been shown to occur in the pathogenesis of cervical cancer, we sought to investigate whether BCL10 possesses clinical significance in relation to cervical cancer. Four cervical cancer cell lines (C33A, SiHa, HeLa, and CaSki) were used in this study. The DNA-binding activity of NF-κB was determined by the luciferase assay. The expression of BCL10, NF-κB, and cyclin D1 in tumor cells from an array of 182 tissue samples was examined using immunohistochemical staining. We transfected four cervical cancer cell lines with BCL10 small interfering RNA (siRNA), and discovered that the down-regulation of BCL10 inhibited the viability of these cervical cancer cells through G1 arrest. BCL10 siRNA treatment inhibited the expression of p-IKKβ and p-IκB, and also down-regulated both NF-κB activation cyclin D1, its downstream cell cycle protein. Our results reveal that cervical cancer had a higher rate of positive cytoplasmic staining (74.1%, 123/166) than either carcinoma in situ (50.0%, 3/6) or normal cervix (0.0%, 0/10); and that poorly differ...Continue Reading

References

Jun 20, 2003·Cell Proliferation·Katrien VermeulenZwi N Berneman
Sep 1, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Sung-Hsin KuoAnn-Lii Cheng
Nov 29, 2005·American Journal of Ophthalmology·Choladda VejabhutiChung-Che Chang
Mar 17, 2010·International Journal of Radiation Oncology, Biology, Physics·Amit K GargThomas A Buchholz
Feb 22, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Sung-Hsin KuoAnn-Lii Cheng

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Citations

Oct 20, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jin XiaQingxia Fan
Apr 2, 2016·Computational and Mathematical Methods in Medicine·Yun-Xia Zhang, Yan-Li Zhao
Nov 20, 2016·Diagnostic Cytopathology·Akinobu OhnoYujiro Asada

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