PMID: 9185159Jul 1, 1997Paper

Expression of beta 1-4 N-acetylgalactosaminyltransferase gene in the developing rat brain and retina: mRNA, protein immunoreactivity and enzyme activity

Neurochemistry International
J L DaniottiH J Maccioni

Abstract

The developmental pattern of expression of the UDP-GalNAc:GM3 N-acetylgalactosaminyltransferase (GalNAc-T) gene was examined in the rat brain and retina. A GalNAc-T cDNA cloned from a rat olfactory bulb cDNA library was used as a probe for Northern blot and in situ hybridization experiments and a rabbit polyclonal antibody to rat GalNAc-T peptide was used for Western blot analysis. In Northern blot experiments, a single approximately 3 kb transcript was detected both in brain and retina. In brain, the abundance of this transcript increased from E15 to PN1-5 and then declined while, in retina, it increased steadily from PN1 to PN13-24. The developmental trends of GalNAc-T mRNA expression, GalNAc-T immunoreactive protein and GalNAc-T activity were comparable in brain. In retina, however, GalNAc-T activity and GalNAc-T peptide immunoreactivity followed developmental patterns that were similar between them and different from that of the specific mRNA. Results suggest that post-transcriptional controls of the GalNAc-T gene expression operate in the rat CNS, which are particularly evident in retina. The expression of the GalNAc-T gene in glial and neuronal cells was examined in rat retina cell cultures by in situ hybridization. The G...Continue Reading

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Citations

Dec 24, 1997·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·J L DaniottiH J Maccioni
Mar 5, 1999·Biochimica Et Biophysica Acta·H J MaccioniJ A Martina
Mar 7, 1998·The Journal of Biological Chemistry·J A MartinaH J Maccioni
Jul 18, 2006·Biochimica Et Biophysica Acta·Gerhild van Echten-Deckert, Thomas Herget

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