Expression of beta 2-integrins and L-selectin on polymorphonuclear leukocytes in septic patients

European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
M ThielK Peter

Abstract

Adhesion molecules on polymorphonuclear leukocytes (PMNL) play an important role in nonspecific defense mechanisms directed at invading microorganisms. When local infection, however, cannot be controlled, a systemic inflammatory response syndrome (SIRS) ensues which may progress to septic shock and multiple organ failure, these being major determinants of the patient's outcome. In the present study, the expression of beta 2-integrins and L-selectin on blood PMNL was measured on subsequent days in patients with sepsis (n = 17) and in healthy volunteers (n = 15). beta 2-Integrins and L-selectin molecules were detected by flow cytometry, using the monoclonal antibodies IB4 (anti-CD18) and Dreg200 (anti-CD62L), respectively. Adhesion molecules were determined at baseline immediately after blood collection and also 45 min after incubation of cells in vitro at body temperature to allow for spontaneous regulation. In addition, PMNL were activated by receptor-dependent and receptor-independent stimuli to characterize stimulus-specific adhesion molecule expression. In parallel with the measurement of adhesion molecules, severity of sepsis was assessed by the Elebute score. The results demonstrate significant differences in the basal, sp...Continue Reading

Citations

Feb 14, 2002·Resuscitation·Timothy H Rainer
May 26, 1999·Infectious Disease Clinics of North America·C Parent, P Q Eichacker
Jul 7, 2005·Tissue Engineering·Christopher J Brinkerhoff, Jennifer J Linderman
Jun 20, 2012·The Journal of Biological Chemistry·Jessica L GiffordHans J Vogel
Mar 17, 2007·Scandinavian Cardiovascular Journal : SCJ·B TraboldD Fröhlich
Jan 18, 2011·Pathology, Research and Practice·P MommsenM van Griensven
Aug 11, 2005·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Tanja BarkhausenMartijn van Griensven
Dec 3, 1999·European Journal of Clinical Investigation·T KernerH Gerlach
May 30, 2019·Frontiers in Immunology·Aleksandar IveticSamuel James Hart
Nov 12, 2021·FEBS Letters·Sascha Rahn, Christoph Becker-Pauly

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