Expression of beta 2 integrins on blood leukocytes of cows with or without bovine leukocyte adhesion deficiency

Veterinary Immunology and Immunopathology
E CoxB M Goddeeris

Abstract

Peripheral blood leukocytes of 11 normal cows, 7 cows heterozygous and 2 heifers homozygous for bovine leukocyte adhesion deficiency (BLAD) were analysed by flow cytometry for the intensity of their beta 2 integrin expression (LFA-1(CD11a/CD18), CR3 (CD11b/CD18) and CR4 (CD11c/CD18)). BLAD-homozygotes revealed no or a very weak expression of the beta 2 integrins and had a 10-fold and 4- to 5-fold increase in absolute number of neutrophils and monocytes, respectively, whereas the absolute number of lymphocytes remained normal. The mean fluorescence intensity (MFI) of the beta 2 integrins (CD18) in heterozygous animals was 56 to 90% of this in the normal cows (MFI between 14 and 512). The difference in the expression level was most pronounced for LFA-1 on the small cluster of lymphocytes with the highest MFI for LFA-1. Repeated analysis and phorbol myristate acetate stimulation revealed that the LFA-1 expression on this high-expressing cell population of the peripheral blood allowed a ready identification of BLAD-heterozygotes by flow cytometry.

References

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Citations

Sep 6, 2000·Veterinary Immunology and Immunopathology·A Diez-FraileC Burvenich
Jan 15, 2010·Veterinary Clinical Pathology·Jeanne W GeorgeV Michael Lane
Jan 13, 2005·The Journal of Veterinary Medical Science·Hajime Nagahata
Jul 21, 2004·The Veterinary Record·T VerfaillieE Cox

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