Expression of beta-adrenergic receptor kinase subtypes in the pregnant rat myometrium

American Journal of Obstetrics and Gynecology
A L Ruzycky, J A DeLoia

Abstract

This study tested the hypothesis that the increase in uterine tachyphylaxis to beta-adrenergic stimulation during pregnancy is associated with increased expression of the beta-adrenergic receptor-inactivating protein kinases. Messenger ribonucleic acid was isolated from snap-frozen myometrium collected from nonpregnant and pregnant rats ranging from 10 to 22 days of gestation. Autoradiographic analysis of beta-adrenergic receptor-inactivating protein kinase messenger ribonucleic acid expression was performed after hybridization with specific complementary deoxyribonucleic acid probes for types 1 and 2 beta-adrenergic receptor-inactivating protein kinases. Probe-specific hybridization was normalized for ribosomal ribonucleic acid detected with methylene blue. Protein expression was detected by Western analysis with use of specific polyclonal antibodies. Myometrial beta-adrenergic receptor-inactivating protein kinase type 2 messenger ribonucleic acid and protein levels increased during the course of pregnancy and in postpartum day 1. In contrast, type 1 levels remained unchanged during the same period. Estrogen treatment resulted in a modest 20% decrease in messenger ribonucleic acid levels of both subtypes. This effect was rever...Continue Reading

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Citations

Jun 30, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Róbert GáspárGeorge Falkay
Aug 27, 2004·American Journal of Physiology. Cell Physiology·Boonsri ChanrachakulRaheela N Khan
Jul 28, 2004·Molecular Reproduction and Development·Anna KlukovitsGeorge Falkay
Jan 14, 2004·The Journal of Pharmacology and Experimental Therapeutics·Kenneth W L KamTak Ming Wong

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