Expression of beta-defensins pBD-1 and pBD-2 along the small intestinal tract of the pig: lack of upregulation in vivo upon Salmonella typhimurium infection

Molecular Immunology
Edwin J A VeldhuizenHenk P Haagsman

Abstract

Defensins are antimicrobial peptides that play an important role in the innate immune response in the intestine. Up to date, only one beta-defensin (pBD-1), has been described in pig, which was found to be expressed at low levels in the intestine. We set-up a quantitative PCR method to detect the gene expression of pBD-1 and a newly discovered porcine beta-defensin, pBD-2. Expression of pBD-1 mRNA increased from the proximal to the distal part of the intestine whereas pBD-2 expression decreased. The main gene expression sites for pBD-2 were kidney and liver, whereas pBD-1 was mainly expressed in tongue. The porcine small intestinal segment perfusion (SISP) technique was used to investigate effects of Salmonella typhimurium DT104 on intestinal morphology and pBD-1 and pBD-2 mRNA levels in vivo. The early responses were studied 2, 4 and 8 h post-infection in four separate jejunal and ileal segments. Immunohistochemistry showed invasion of the mucosa by Salmonella and changes in intestinal morphology. However, no concomitant changes in expression of either pBD-1 or pBD-2 were observed. We conclude that at least two defensins are differentially expressed in the intestine of pigs, and that expression of both defensins is not altered...Continue Reading

References

Dec 1, 1989·The Journal of Clinical Investigation·M C TerritoR Lehrer
Apr 1, 1995·Molecular and Biochemical Parasitology·D E JonesD P Clark
Jul 1, 1993·Research in Veterinary Science·M J NabuursJ D van der Klis
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·G DiamondC L Bevins
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·E D StolzenbergM Zasloff
Dec 29, 1999·Nature Medicine·J LiM Simons
Nov 22, 2000·The Journal of Biological Chemistry·J HarderJ M Schroder
Jan 17, 2002·Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan·F LiuT Kaku
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·A GiuliettiC Mathieu
Aug 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jian Xu, Jeffrey I Gordon
Sep 13, 2003·Developmental and Comparative Immunology·David K MeyerholzMark R Ackermann
May 21, 2004·Nature Reviews. Microbiology·B Brett Finlay, Robert E W Hancock
Jan 25, 2005·Emerging Infectious Diseases·Susan W M HendriksenEngeline van Duijkeren
Nov 3, 2005·Nature Clinical Practice. Gastroenterology & Hepatology·Jan WehkampEduard F Stange
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jan WehkampCharles L Bevins
May 1, 2002·Meat Science·M H G TersteegF van Knapen

❮ Previous
Next ❯

Citations

Apr 19, 2011·Animal Biotechnology·Yinfeng YangShuying Liu
Mar 4, 2008·Journal of Veterinary Internal Medicine·A LindeT Melgarejo
Sep 2, 2008·Developmental and Comparative Immunology·Mick Bailey
Jun 20, 2014·Luminescence : the Journal of Biological and Chemical Luminescence·Xi YangYonggang Hu
Jul 31, 2007·Molecular Immunology·Edwin J A VeldhuizenHenk P Haagsman
Jan 21, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Feifei HanYizhen Wang
May 24, 2019·Avian Diseases·Erik P H de LeeuwHyun S Lillehoj
Feb 15, 2019·Frontiers in Immunology·Kieran G Meade, Cliona O'Farrelly
Oct 16, 2012·Molecular Biology Reports·Xiangbing MaoDaiwen Chen
Jul 31, 2019·Journal of Animal Science and Biotechnology·Guoqi SuJun He
Mar 27, 2020·Critical Reviews in Food Science and Nutrition·Murphy L Y WanHani El-Nezami
May 26, 2021·Peptides·C E Valdez-MiramontesB Rivas-Santiago

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.