Expression of BK(Ca) channels in human pulmonary arteries: relationship with remodeling and hypoxic pulmonary vasoconstriction

Vascular Pharmacology
Víctor I PeinadoJoan A Barberà

Abstract

Potassium channels are important in pulmonary circulation because they have been closely related to hypoxic pulmonary vasoconstriction (HPV). The objective of the study was to determine whether structural changes in pulmonary arteries (PA), such as those observed in patients with chronic obstructive pulmonary disease (COPD), might be associated with changes in the mRNA expression of both BK(Ca) and K(V) channels and their potential relationship with HPV. PA (about 1.5 mm in diameter) were obtained from 16 patients who underwent resective lung surgery. Intimal thickening was evaluated morphometrically. mRNA expression of BK(Ca), K(V)1.2, K(V)1.5, K(V)2.1 and K(V)3.1 was evaluated by RT-PCR in PA homogenates. Endothelial function and HPV were assessed in vitro in isolated PA using an organ bath. Intimal enlargement was closely associated with an increase in the expression of BK(Ca) channel (r=0.57, p<0.05). Pulmonary arteries incubated with charybdotoxin, a BK(Ca) channel blocking agent, showed lower response to endothelium-dependent vasodilators indicating its contribution to reduce vascular tone. Pulmonary arteries with more pronounced responses to hypoxia were those with greater gene expression of BK(Ca) channels, suggesting a...Continue Reading

References

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Sep 6, 2002·The European Respiratory Journal·V I PeinadoJ A Barberà
Oct 16, 2002·Vascular Pharmacology·Mehran Mandegar, Jason X J Yuan

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Citations

Sep 12, 2013·BioMed Research International·Priyadharshanan AriyaratnamAlyn H Morice
Mar 8, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Carla Blum-JohnstonSean M Wilson
Feb 22, 2017·The Open Cardiovascular Medicine Journal·A HussainA Morice
Oct 15, 2011·Journal of Cardiovascular Pharmacology·Hui XuGregory D Fink
Oct 25, 2011·American Journal of Respiratory Cell and Molecular Biology·Yingji LiAndrea Olschewski
Feb 3, 2012·Physiological Reviews·J T SylvesterJeremy P T Ward
Dec 12, 2020·American Journal of Respiratory and Critical Care Medicine·Javier Sevilla-MonteroMaría J Calzada
May 18, 2010·Vascular Pharmacology·Alexander VangGaurav Choudhary

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