Expression of BKV and JCV encoded microRNA in human cerebrospinal fluid, plasma and urine

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Tuuli PietiläEeva Auvinen

Abstract

BK and JC polyomaviruses encode microRNAs which may facilitate the establishment of persistent infection. MicroRNAs contribute to disease pathogenesis, and may provide useful tools in laboratory diagnostics and patient management. In this pilot work we studied whether viral and cellular microRNAs can be extracted and detected from body fluids to provide added value in a diagnostic laboratory. Altogether 120 human plasma, urine, and cerebrospinal fluid samples from individuals diagnosed with, or suspected of, a severe polyomavirus associated disease, were included in the study. The samples were spiked with unrelated synthetic microRNA to control for sample quality and inhibition. BKV specific bkv-miR-B1-5p, JCV specific jcv-miR-J1-5p, and bkv-miR-B1-3p/jcv-miR-J1-3p, sharing identical sequences between the two viruses, were amplified from human samples using specific TaqMan assays. Expression of 84 circulating human microRNAs was studied in four selected plasma samples in microarray. jcv-miR-J1-5p and bkv-miR-B1-3p/jcv-miR-J1-3p were frequently amplified from human plasma, urine, and cerebrospinal fluid samples. bkv-miR-B1-5p was amplified from one-third of the samples, which often contained high viral DNA loads. A microarray sc...Continue Reading

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Citations

Sep 30, 2016·Molecular Diagnosis & Therapy·Eeva Auvinen
Oct 27, 2016·Viruses·Heidi BarthSamira Fafi-Kremer
Sep 16, 2017·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Eric M L Williamson, Joseph R Berger
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Mar 16, 2017·Reviews in Medical Virology·Francesco Martelli, Simone Giannecchini
Aug 15, 2019·Transplant Infectious Disease : an Official Journal of the Transplantation Society·Gang ZengParmjeet Randhawa
Oct 22, 2020·Molecular Biology Reports·Simone AgostiniMario Clerici
Aug 20, 2019·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Yuchen HuangParmjeet S Randhawa
Apr 3, 2021·Journal of Oral Microbiology·Ana Carolina MamanaSimone Giannecchini
Apr 24, 2021·Frontiers in Microbiology·Wei Zou, Michael J Imperiale

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