Expression of bone morphogenetic protein in human adenocarcinoma cell line

Biochemical and Biophysical Research Communications
S HatakeyamaM Satoh

Abstract

A subclone termed as HSG-S8 was previously isolated from human salivary adenocarcinoma cell line, HSG, and proliferated in a serum-free culture. When HSG-S8 and parental HSG cells were transplanted into nude mice i.m. or s.c., both cells reproducibly induced adenocarcinoma. Furthermore, injection of HSG-S8 consistently induced formation of cartilage i.m. or bone s.c. in tumors, but did not parental HSG cells. The cytoplasm of HSG-S8 cells was specifically immunostained by anti-bone morphogenetic protein (BMP)-2 antibody. The conditioned medium of HSG-S8 cells contained a protein of M(r) 18,000 which specifically reacted with anti-BMP-2 protein antibody. Northern blot analysis also revealed that HSG-S8 cells expressed transcripts for BMP-2. On the other hand, parental HSG cells gave a very slightly positive signal only in Northern blot analysis. These results indicate that HSG-S8 cells synthesize and secrete BMP-2 protein, which is probably involved in the formation of cartilage and bone in the tumor tissues in nude mice.

Citations

May 1, 1994·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·S HatakeyamaT Otsu
Apr 1, 1995·In Vitro Cellular & Developmental Biology. Animal·T NaritaH Kudo
Jan 1, 1995·DNA Sequence : the Journal of DNA Sequencing and Mapping·S OidaS Sasaki
Sep 2, 1998·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·M ZhaoH Nikai
Aug 15, 2015·Spine·Branko SkovrljSamuel K Cho
May 25, 2010·International Journal of Laboratory Hematology·Justyna DzietczeniaK Kuliczkowski
Jun 20, 2012·FEBS Letters·Jie CaiPeter ten Dijke
Sep 6, 2011·Journal of Cancer Research and Clinical Oncology·Ke WangYan Zhang
May 1, 1995·Pathology International·T NaritaS Yokoyama
Jan 1, 1993·Virchows Archiv. A, Pathological Anatomy and Histopathology·Y LianjiaM Masahiko
Oct 19, 2007·International Journal of Cancer. Journal International Du Cancer·Christian BuschMatthias Oppitz
May 12, 2009·The Journal of Pathology·Ulrike MaegdefrauAnja-Katrin Bosserhoff
May 23, 2015·Acta Biochimica Et Biophysica Sinica·Taoran DengXiangning Fu
Feb 1, 1997·Experimental Cell Research·L NissinenJ Heino
Jan 16, 2010·International Journal of Oral and Maxillofacial Surgery·Q GaoP H Krebsbach
Aug 7, 2010·Cytokine & Growth Factor Reviews·Ashok Singh, Rebecca J Morris

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.