Expression of bone sialoprotein mRNA during bone formation and resorption induced by colchicine in rat tibial bone marrow cavity

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
N AraiT Amagasa

Abstract

In the rat tibial bone marrow cavity, following colchicine injection, there is a phase of osteogenesis in which bone trabeculae replace the necrotic bone marrow tissues and fill the marrow cavity. The newly formed bone is subsequently resorbed by osteoclasts and normal bone marrow is restored. In this study, we correlated these morphologic events with the pattern of gene expression of bone sialoprotein (BSP), an extracellular matrix protein in mineralized tissues, to elucidate the possible functions of BSP in bone formation and resorption in vivo. The expressions of osteopontin (OPN) and type I collagen were also examined. Northern hybridization of the tibia demonstrated that OPN mRNA was gradually increased and expressed at a maximal level 10 days after colchicine injection (during the bone resorption process), while BSP mRNA expression already reached a maximal level at day 6 (during the initial process of bone formation). Its expression was, thus, quite temporary at the beginning of bone formation and different from that of type I collagen, which was continually elevated from days 6 to 10. In situ hybridization of the newly formed bone induced in the tibia revealed that BSP mRNA was evenly expressed in most osteoblasts and o...Continue Reading

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Citations

Sep 24, 2010·PloS One·Karen FullerTim J Chambers
Mar 20, 2008·The American Journal of Pathology·Sasanka RamanadhamJohn Turk
Sep 3, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Maya BoudiffaLuc Malaval
Apr 29, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L F CooperS W Whitson
Jan 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N SudaV E Hammond
Dec 14, 1999·Calcified Tissue International·G CaselliP Bianco
Dec 17, 2011·Annals of the New York Academy of Sciences·T J Chambers, K Fuller
Feb 14, 2019·Brazilian Oral Research·Hulya TokerFikret Gevrek
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B GanssJ Sodek
Mar 2, 1999·Biochemical and Biophysical Research Communications·W R DuarteS Kasugai

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