Expression of botulinum toxin binding sites in Xenopus oocytes.

Infection and Immunity
N BakryL L Simpson

Abstract

The binding of iodinated botulinum toxin type B to nerve membranes was studied by using rat and mouse preparations. The toxin was examined both in the single-chain and in the proteolytically processed dichain form, and binding sites both in the spinal cord and in various brain regions were assayed. Rat and mouse brains possessed specific binding sites for botulinum toxin type B. The average Kd values for the various rat and mouse membrane preparations examined were 4.2 +/- 0.7 nM and 3.7 +/- 0.9 nM, respectively. The average Bmax values for the same tissue preparations were 7.3 +/- 0.7 pmol/mg of protein and 7.5 +/- 1.9 pmol/mg protein, respectively. The binding of botulinum toxin type B to rat brain membranes was not antagonized by a polyclonal antibody against the cytosolic domain of synaptotagmin 1 or by a monoclonal antibody directed against the luminal domain of synaptotagmin 1. In addition, these antibodies did not protect the mouse phrenic nerve-hemidiaphragm from toxin-induced neuromuscular blockade. Extraction of whole-brain mRNA and injection into Xenopus oocytes led to expression of binding sites for botulinum toxin. Extraction and injection of cerebellar mRNA led to expression of a higher density of binding sites. T...Continue Reading

References

Jul 31, 1979·Naunyn-Schmiedeberg's Archives of Pharmacology·S Kozaki
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·N YokosawaK Oguma
Jan 1, 1988·Methods in Enzymology·L L SimpsonJ L Middlebrook
Jan 1, 1982·Pharmacology & Therapeutics·G Sakaguchi
Jul 1, 1994·Molecular Microbiology·C Montecucco, G Schiavo
Mar 9, 1994·Annals of the New York Academy of Sciences·G SchiavoC Montecucco
Apr 29, 1994·Biochemical and Biophysical Research Communications·S YamasakiH Niemann
Aug 12, 1993·Nature·T PatarnelloC Montecucco
Aug 1, 1949·The Journal of Physiology·A S V BURGENL J ZATMAN

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Citations

Jan 5, 2002·Journal of Physiology, Paris·Frédéric A MeunierJordi Molgó
Aug 21, 2003·Annales de réadaptation et de médecine physique : revue scientifique de la Société française de rééducation fonctionnelle de réadaptation et de médecine physique·B Poulain, Y Humeau
Sep 19, 2003·Trends in Microbiology·Giovanna LalliGiampietro Schiavo
Mar 14, 2002·International Journal of Medical Microbiology : IJMM·Judit Herreros, Giampietro Schiavo
Dec 8, 2011·Biochemistry·Behzod Z DolimbekM Zouhair Atassi

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