Expression of c-fos gene inhibits proteoglycan synthesis in transfected chondrocyte

FEBS Letters
M TsujiT Yoshida

Abstract

The effect of expression of c-fos gene on proteoglycan synthesis, one of the important markers of cartilage metabolism, was examined by introducing the c-fos DNA into HCS 2/8 chondrocytes. The [35S]sulfate incorporation into proteoglycan was decreased in the c-fos transfectants expressing exogenous c-fos mRNA, when compared to a control transfectant. A significant increase in transcription of MMP-3 with the suppressed transcription of aggrecan and TIMP-1 were also observed in the c-fos transfectants. Moreover, analysis of the effect of AP-1 proteins on the collagenase and TIMP-1 promoters in gastric carcinoma KKLS cells revealed that c-Fos combined with any of the Jun-related proteins failed to stimulate the TIMP-1 promoter, though collagenase promoter was effectively activated by any Fos/Jun-related protein heterocomplex. These findings indicate that the c-fos expression may govern the cartilage metabolism and hence may play an important role in the pathogenesis of joint destruction in arthritis.

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Citations

Apr 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K SuzukiR S Bockman
Sep 12, 2013·PloS One·Katsuya YokoyamaJoji Mochida
Mar 1, 2001·Modern Rheumatology·M TsujiK Fujii
Oct 30, 2001·Osteoarthritis and Cartilage·M Enomoto-IwamotoM Iwamoto
Oct 27, 2004·Osteoarthritis and Cartilage·J SaasT Aigner
Oct 29, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T MajimaD A Hart
Nov 8, 2003·World Journal of Gastroenterology : WJG·Ai-Hua XuLing-Chang Jia
Jan 23, 1997·Biochemical and Biophysical Research Communications·P A ScherleE C Arner

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