Expression of c-Kit receptor mRNA and protein in the developing, adult and irradiated rodent testis

Reproduction : the Official Journal of the Society for the Study of Fertility
Sridurga Mithra PrabhuKate Lakoski Loveland

Abstract

Germ cell proliferation, migration and survival during all stages of spermatogenesis are affected by stem cell factor signalling through the c-Kit receptor, the expression and function of which are vital for normal male reproductive function. The present study comprehensively describes the c-Kit mRNA and protein cellular expression profiles in germ cells of the postnatal and adult rodent testis, revealing their significant elevation in synthesis at the onset of spermatogenesis. Real-time PCR analysis for both mice and rats matched the cellular mRNA expression profile where examined. Localization studies in normal mouse testes indicated that both c-Kit mRNA and protein are first detectable in differentiating spermatogonia. In addition, all spermatogonia isolated from 8-day-old mice displayed detectable c-Kit mRNA, but 30-50% of these lacked protein expression. The c-Kit mRNA and protein profile in normal rat testes indicated expression in gonocytes, in addition to differentiating spermatogonia. However, in the irradiated adult rat testes, in which undifferentiated spermatogonia are the only germ cell type, mRNA was also detected in the absence of protein. This persisted at 3 days and 1 and 2 weeks following treatment with gonado...Continue Reading

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Citations

Mar 15, 2008·Cell and Tissue Research·Geraldine LoppionPascal Sourdaine
Nov 30, 2013·Reproductive Biology·Kamila MisiakiewiczBarbara Wiszniewska
May 12, 2010·Expert Reviews in Molecular Medicine·Roopa L Nalam, Martin M Matzuk
Feb 5, 2010·Biology of Reproduction·Sridurga MithraprabhuKate L Loveland
May 14, 2010·Reproductive Biology and Endocrinology : RB&E·Zhao-juan YangJi Wu
May 14, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Sreepoorna K UnniDeepa Bhartiya
Jul 26, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Amanda V AlbuquerqueHélio Chiarini-Garcia
Oct 15, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Adrien BosseboeufPascal Sourdaine
Jul 2, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Sridurga Mithraprabhu, Kate L Loveland
May 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Nicole A SiddallGary R Hime
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Mar 10, 2016·Seminars in Cell & Developmental Biology·Carla BoitaniElena Vicini
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Jun 16, 2009·Microscopy Research and Technique·Catherine ItmanKate Lakoski Loveland
May 4, 2012·Spermatogenesis·Damien HunterRichard Ivell
Feb 10, 2012·Spermatogenesis·Lei ZhangYibing Han
Dec 3, 2014·Developmental Biology·Jonathan T BusadaChristopher B Geyer
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Nov 15, 2013·Biology of Reproduction·Catriona PaulBernard Robaire
Apr 25, 2017·Molecular Reproduction and Development·Amir FattahiMohammad Nouri

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