PMID: 16502487Feb 28, 2006Paper

Expression of Ca2+-dependent synaptotagmin isoforms in mouse and rat parotid acinar cells

Yonsei Medical Journal
Hae JoHeung-Kyu Son

Abstract

Synaptotagmin is a Ca2+ sensing protein, which triggers a fusion of synaptic vesicles in neuronal transmission. Little is known regarding the expression of Ca2+-dependent synaptotagmin isoforms and their contribution to the release of secretory vesicles in mouse and rat parotid acinar cells. We investigated a type of Ca2+-dependent synaptotagmin and Ca2+ signaling in both rat and mouse parotid acinar cells using RT-PCR, microfluorometry, and amylase assay. Mouse parotid acinar cells exhibited much more sensitive amylase release in response to muscarinic stimulation than did rat parotid acinar cells. However, transient [Ca2+]i increases and Ca2+ influx in response to muscarinic stimulation in both cells were identical, suggesting that the expression or activity of the Ca2+ sensing proteins is different. Seven Ca2+-dependent synaptotagmins, from 1 to 7, were expressed in the mouse parotid acinar cells. However, in the rat parotid acinar cells, only synaptotagmins 1, 3, 4 and 7 were expressed. These results indicate that the expression of Ca2+-dependent synaptotagmins may contribute to the release of secretory vesicles in parotid acinar cells.

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Citations

Jun 4, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michelle A FalkowskiGuy E Groblewski
Apr 20, 2014·Cell Calcium·Scott W MessengerGuy E Groblewski
May 26, 2018·The Journal of General Physiology·Daniel D MacDougallArun Anantharam
Oct 20, 2018·International Journal of Molecular Sciences·Akiko Suzuki, Junichi Iwata

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