Expression of calcification-related ion transporters during blue mussel larval development

Ecology and Evolution
Kirti RameshFrank Melzner

Abstract

The physiological processes driving the rapid rates of calcification in larval bivalves are poorly understood. Here, we use a calcification substrate-limited approach (low dissolved inorganic carbon, CT) and mRNA sequencing to identify proteins involved in bicarbonate acquisition during shell formation. As a secondary approach, we examined expression of ion transport and shell matrix proteins (SMPs) over the course of larval development and shell formation. We reared four families of Mytilus edulis under ambient (ca. 1865 µmol/kg) and low CT (ca. 941 µmol/kg) conditions and compared expression patterns at six developmental time points. Larvae reared under low CT exhibited a developmental delay, and a small subset of contigs was differentially regulated between ambient and low CT conditions. Of particular note was the identification of one contig encoding an anion transporter (SLC26) which was strongly upregulated (2.3-2.9 fold) under low CT conditions. By analyzing gene expression profiles over the course of larval development, we are able to isolate sequences encoding ion transport and SMPs to enhance our understanding of cellular pathways underlying larval calcification processes. In particular, we observe the differential ex...Continue Reading

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Citations

Aug 2, 2020·Biological Reviews of the Cambridge Philosophical Society·Melody S ClarkElizabeth M Harper
Aug 21, 2020·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Kirti RameshNina Himmerkus
Feb 11, 2020·Frontiers in Physiology·J Kirsikka SillanpääKristina Sundell
May 7, 2020·Scientific Reports·João C R CardosoDeborah M Power
May 31, 2020·The Journal of Experimental Biology·Melody S Clark
Feb 1, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Jie LiuXiwu Yan
Apr 15, 2021·Marine Environmental Research·Laura Emilia Fernández-GonzálezAngel P Diz
May 9, 2021·Global Change Biology·Kanmani Chandra RajanThiyagarajan Vengatesen
May 21, 2021·Molecular Biology and Evolution·Tejaswi YarraMelody S Clark
Jan 11, 2020·The Science of the Total Environment·Liqiang ZhaoYuewen Deng

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Datasets Mentioned

BETA
PRJNA494236

Methods Mentioned

BETA
transfection

Software Mentioned

Interproscan
Trinity pipeline
ProgRes Capture Pro
mcf
RSEM
BLAST
Trimmomatic
CO2SYS
Fastq
Transdecoder

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