Expression of cannabinoid receptor mRNA in murine and human leukocytes

Advances in Experimental Medicine and Biology
Y DaakaH Friedman

Abstract

delta 9-tetrahydrocannabinol, the major psychoactive cannabinoid of marijuana modulates immune cells in vivo and in vitro. It is possible that the drug exerts it's effect either by inserting into and disrupting the cell membrane (nonreceptor mechanism) or by binding to a cannabinoid receptor moiety and thus altering cell function through some form of signal transduction. In the present study, we confirm and extend the findings that mouse and human immune cells express specific cannabinoid binding sites and cannabinoid receptor mRNA. Reverse transcription-polymerase chain reaction analysis showed the presence of receptor mRNA not only in the neuroblastoma cell line (N18TG-2), but also in mouse splenocytes and in cell lines such as NKB61A2 (a mouse natural killer-like), CTLL2 (a mouse IL2-dependent T cell), THP-1 (a human monocytic cell) and Raji (a human B cell) but not in Jurkat (a human T cell). Furthermore, the receptor mRNA was expressed in purified populations of resting splenic T and B lymphocytes but not in resting populations of enriched splenic macrophages. Finally, LPS-stimulated Raji and PMA-stimulated THP-1 human cell lines showed increased levels of the cannabinoid receptor mRNA. These results suggest cannabinoid re...Continue Reading

Citations

Oct 17, 2006·Journal of Leukocyte Biology·Christine BörnerJürgen Kraus
Nov 14, 1997·Clinical Reviews in Allergy & Immunology·B E Van Hoozen, C E Cross
May 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria-Teresa SamsonHelen Turner
Jan 27, 2005·The Biochemical Journal·Andrea L Small-HowardHelen Turner
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Guy A Cabral
Nov 27, 2007·British Journal of Pharmacology·G A CabralF Marciano-Cabral
Sep 7, 2016·Mediators of Inflammation·Kathleen L McCoy

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