Expression of carbonic anhydrase IX in mouse tissues

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Mika HilvoSeppo Parkkila

Abstract

Carbonic anhydrase IX (CA IX) is a unique member of the CA gene family. In contrast to the other isozymes, it has been implicated in regulation of cell proliferation, adhesion, and malignant cell invasion. In a recently described knockout mouse model for CA IX deficiency, the only phenotypic abnormalities were limited to the gastric mucosa, while no changes were observed in the other tissues known to express CA IX in rats and humans. Here we investigated the expression of CA IX mRNA and protein in mouse tissues. Immunohistochemical (IHC) analysis showed strong staining in the gastric mucosa. Moderate reactions were seen in the colon enterocytes and pancreatic acini. The expression pattern of CA IX was similar in certain human and rodent tissues, although some differences existed, especially in the gut epithelium. Reverse transcriptase PCR analyses surprisingly revealed strong signals for CA IX mRNA in the kidney and skeletal muscle, while the IHC and Western blotting showed no or weak signals for the corresponding protein. This result suggests a tight tissue-specific post-transcriptional control for CA IX expression, possibly related to the physiological demands.

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Citations

Oct 19, 2004·Reproductive Biology and Endocrinology : RB&E·Piritta HynninenSeppo Parkkila
May 25, 2006·BMC Developmental Biology·Heini KallioSeppo Parkkila
Aug 23, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Renate J ScheibePetra Wetzel
Mar 17, 2007·BMC Molecular Biology·Pei-wen PanSeppo Parkkila
Feb 27, 2013·PloS One·Toshifumi MaruyamaRyutaro Kamijo
Mar 16, 2013·Expert Opinion on Therapeutic Patents·Fabrizio Carta, Claudiu T Supuran
Jul 19, 2012·Journal of Enzyme Inhibition and Medicinal Chemistry·Anna-Kaisa HarjuSeppo Parkkila
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Dec 18, 2009·Journal of Cellular and Molecular Medicine·Johanna ChicheJacques Pouysségur
Aug 16, 2008·The Journal of Biological Chemistry·Mika HilvoSeppo Parkkila
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