Expression of cartilage developmental genes in Hoxc8- and Hoxd4-transgenic mice.

PloS One
Claudia Kruger, Claudia Kappen

Abstract

Hox genes encode transcription factors, which regulate skeletal patterning and chondrocyte differentiation during the development of cartilage, the precursor to mature bone. Overexpression of the homeobox transcription factors Hoxc8 and Hoxd4 causes severe cartilage defects due to delay in cartilage maturation. Matrix metalloproteinases (MMPs), bone morphogenetic proteins (BMPs) and fibroblastic growth factors (FGFs) are known to play important roles in skeletal development and endochondral bone formation and remodeling. In order to investigate whether these molecules are aberrantly expressed in Hoxc8- and/or Hoxd4-transgenic cartilage, we performed quantitative RT-PCR on chondrocytes from Hox-transgenic mice. Gene expression levels of Bmp4, Fgf8, Fgf10, Mmp9, Mmp13, Nos3, Timp3, Wnt3a and Wnt5a were altered in Hoxc8-transgenic chondrocytes, and Fgfr3, Ihh, Mmp8, and Wnt3a expression levels were altered in Hoxd4-transgenic chondrocytes, respectively. Notably, Wnt3a expression was elevated in Hoxc8- and reduced in Hoxd4-transgenic cartilage. These results suggest that both transcription factors affect cartilage maturation through different molecular mechanisms, and provide the basis for future studies into the role of these gene...Continue Reading

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Oct 17, 2013·International Journal of Molecular Sciences·Pierre M L DeprezCatherine Nyssen-Behets
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Jul 1, 2010·Cartilage·Claudia Kruger, Claudia Kappen

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Methods Mentioned

BETA
transgenic
genotyping
dissection
PCR
transgenics

Software Mentioned

GraphPad PRISM®
ABI Prism 7000 SDS
GraphPad
ENSEMBL
Data Analysis Pack
Excel X
ENSEMBLE browser

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