PMID: 15348168Sep 7, 2004Paper

Expression of caspase-8 and caspase-3 proteins in interface membranes from aseptically loose total hip arthroplasty

Journal of Materials Science. Materials in Medicine
A PetitO L Huk

Abstract

The terminal events leading to periprosthetic osteolysis are multifactorial and modulation of this process after the stage of mediator release has been demonstrated futile. We demonstrated that ceramic induce macrophage apoptosis in vitro. More recently, we and others demonstrated the presence of apoptosis in interface membranes (IMs) from aseptically loose total hip arthroplasty (THA). The purpose of this study was to characterize the mechanisms leading to apoptosis in these pseudomembranes. Western blot analysis was used to characterize the expression of caspase-3, caspase-8, poly(ADP-ribose)polymerase (PARP), and p53 proteins in IM from 35 patients (40 specimens) with a mean age of 58 years (range, 28-88 years) at the time of revision. Tissue harvested at the time of routine hardware removal served as control. Our results show that caspase-3 and caspase-8 pro-enzymes were expressed in both control and IM tissues. Our results also showed that caspase-3 active fragment (17 kDa) as well as caspase-8 active fragment (18 kDa) were expressed in IMs but absent in control tissues. We also demonstrated that both the native PARP (113 kDa) and its proteolytic fragment (89 kDa) were present in osteolytic IMs. Control membranes expressed...Continue Reading

Citations

Mar 28, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Guoyin LiuJianning Zhao
Feb 1, 2006·Biomaterials·Stefan LandgraeberMarius von Knoch

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