PMID: 8733297Apr 26, 1996

Expression of cathepsin D during the progression of human gliomas

Neuroscience Letters
M SivaparvathiJ S Rao

Abstract

Recent studies suggest that aspartic proteinase cathepsin D may be implicated in tumor invasion and metastasis either directly by degrading extracellular matrix or indirectly by activating the cysteine proteinases such as procathepsin B, H, and L to mature forms or by inactivating cysteine proteinase inhibitors. In this study we determined for the first time whether increased levels of cathepsin D correlate with glioma progression by enzymatic assay, ELISA, and western blotting. Cathepsin D activity and content were higher in anaplastic astrocytoma and in glioblastoma tissue extracts especially when compared to normal brain tissue and low-grade gliomas. There was a significantly increased intensity of an M(r) 29,000 band in glioblastoma and anaplastic astrocytoma compared to low-grade glioma and normal brain tissue on Western blotting analysis using its specific antibodies. Cathepsin D antibody inhibited the invasion of glioblastoma cell lines in a dose-dependent manner. These results suggest that the expression of cathepsin D is dramatically upregulated in malignant gliomas, and that its increase correlates with the malignant progression of human gliomas in vivo.

References

Feb 1, 1990·Neuropathology and Applied Neurobiology·D K RobsonP R Bogue
Jan 1, 1986·Annual Review of Biochemistry·K von Figura, A Hasilik
Jan 1, 1988·The International Journal of Biochemistry·G LetoL Rausa
Jul 29, 1988·Biochemical and Biophysical Research Communications·B LenarcicV Turk
Jan 1, 1995·Clinical & Experimental Metastasis·M SivaparvathiJ S Rao
Sep 20, 1938·The Journal of General Physiology·M L Anson

Citations

Jan 1, 1997·Brain Tumor Pathology·K IiK Matsumoto
Apr 20, 2004·The International Journal of Biochemistry & Cell Biology·Anita C BellailErwin G Van Meir
Apr 20, 2011·The Journal of Organic Chemistry·Zhibin LiangPhilip G Williams
Mar 1, 2006·Expert Opinion on Biological Therapy·Tamara T LahCornelis J F Van Noorden
Nov 22, 2000·Pathology, Research and Practice·D S Tews
Apr 9, 2008·Critical Reviews in Oncology/hematology·Petr BenesMartin Fusek
Mar 28, 2006·Pathology, Research and Practice·A Josefine U von RandowD S Tews
Dec 17, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Monika Paul-SamojednyJan Kowalski
Jul 9, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jingxin QiuKeith D Robertson
Nov 1, 2007·Proteomics. Clinical Applications·Joshua K McBeeRichard S Morrison
Jan 11, 2003·American Journal of Clinical Pathology·Elias A CastillaMark L Cohen
Apr 2, 2004·Journal of Neuropathology and Experimental Neurology·Lars TatenhorstWerner Paulus
Oct 23, 2009·Journal of Neuro-oncology·Adrianna RangerRolando Delmaestro
Apr 6, 2019·Frontiers in Surgery·Rosanna M A RahmanTinte Itinteang
Jun 15, 2017·Frontiers in Surgery·Sabrina P KohTinte Itinteang
Jun 17, 2005·Cancer Research·Mieko E FukudaNaohiko Seki

Related Concepts

Brain
Brain Tumor, Recurrent
Cathepsin D
Mixed Gliomas
Western Blot

Related Feeds

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.