Expression of CD105 and CD34 receptors controls BMP-induced in vitro mineralization of mouse adipose-derived stem cells but does not predict their in vivo bone-forming potential

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Vedavathi MadhuQuanjun Cui

Abstract

Adipose-derived stem cells (ADSCs) can be excellent alternative to bone marrow derived stem cells for enhancing fracture repair since ADSCs can be isolated comparatively in large numbers from discarded lipoaspirates. However, osteogenic potential of ADSCs in vivo is very controversial. We hypothesized that adipose-derived stem cells (ADSCs) that respond maximally to bone morphogenetic proteins (BMPs) in vitro would possess maximum bone-forming potential. Four purified populations of mouse ADSCs: CD105(+) CD34(+), CD105(-) CD34(-), CD105(+) CD34(-) and CD105(-) CD34(+) were obtained using fluorescence-activated cell sorting (FACS) and their BMP-responsiveness was determined in vitro. CD105(+) CD34(-) population showed the strongest response to BMPs in terms of robust increase in mineralization. Expression of CD105 correlated with high BMP-responsive phenotype and larger cell size while expression of CD34 correlated with low BMP-responsive phenotype and smaller cell size. CD105(+) CD34(-) population displayed higher gene expression of Alk1 or Alk6 receptors in comparison with other populations. However, CD105(+) CD34(-) ADSCs failed to induce ectopic bone formation in vivo after they were transplanted into syngeneic mice, indicat...Continue Reading

References

Oct 15, 1993·Science·S BaumheterL A Lasky
Jan 1, 1993·The Journal of Bone and Joint Surgery. American Volume·D R DiduchG Balian
Jul 5, 2005·The Journal of Bone and Joint Surgery. American Volume·Ph HernigouH Rouard
May 18, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Takahiro NiikuraA Hari Reddi
Jul 15, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Per AspenbergMark C Lakshmanan
Jul 15, 2009·Clinical Orthopaedics and Related Research·Marc FajardoKenneth Egol
Oct 17, 2012·Cytotherapy·Ching-Shwun LinTom F Lue
Dec 1, 2012·Stem Cells Translational Medicine·Aaron W JamesChia Soo
Dec 12, 2012·Tissue Engineering. Part a·Michael T ChungDerrick C Wan
Jan 19, 2013·Journal of Applied Oral Science : Revista FOB·Ariadne Cristiane Cabral CruzCláudia Maria Oliveira Simões
Apr 8, 2014·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·E KoellenspergerU Leimer
Apr 17, 2014·Journal of Biomedical Materials Research. Part a·Jinling MaJeroen J J P van den Beucken
Jul 2, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ruei-Zeng LinJuan M Melero-Martin

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