PMID: 9171507Dec 1, 1996Paper

Expression of CD1a monocytes cultured with supernatants from periodontally diseased gingival epithelial cells

Oral Diseases
T A WinningL J Walsh

Abstract

Langerhans cells are believed to originate from the monocyte lineage and have been reported to increase in number with plaque accumulation and gingival inflammation. The aim of this study was to investigate the effects of local gingival epithelial factors on the induction of CD1a, a Langerhans cell phenotype, on monocyte rich populations. Peripheral blood monocyte rich in populations from healthy subjects were cultured for 24 h with either healthy gingival or periodontally diseased gingival epithelial supernatants. Additionally, the monocyte rich populations were cultured with cytokines IL-I alpha, IL-I beta, IL-6 and TNF-alpha which are known to be produced by epithelial cells or co-cultured with autologous epithelial cells. The per cent CD1a positive cells was determined using FACS analysis. Healthy gingival supernatants did not induce CD1a expression in monocyte rich populations, however, a significant increase in per cent CD1a+ cells for monocyte rich populations cultured with five (P < 0.01) of six periodontal gingival epithelial supernatants was found. IL-I alpha or TNF-alpha (10 ng/well) resulted in a significant increase in the per cent CD1a+ cells (P < 0.01). Depletion of CD1a+ Langerhans cells from healthy gingival ep...Continue Reading

References

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Citations

Nov 22, 2013·Journal of Clinical Periodontology·Jaime Díaz-ZúñigaRolando Vernal
Jan 6, 2004·Journal of Biomedical Materials Research. Part a·Stéphane LamarqueMartine Bonnaure-Mallet
Apr 27, 2004·Periodontology 2000·Christopher W Cutler, Ravi Jotwani
Jul 25, 2006·Journal of Dental Research·C W Cutler, R Jotwani
Nov 25, 2004·Cytokine & Growth Factor Reviews·Hanna SurawskaRavi Salgia

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