Expression of CD24 in Human Bone Marrow-Derived Mesenchymal Stromal Cells Is Regulated by TGFβ3 and Induces a Myofibroblast-Like Genotype

Stem Cells International
Luisa Marilena SchäckSandra Noack

Abstract

Human bone marrow-derived stromal cells (hBMSCs) derived from the adult organism hold great promise for diverse settings in regenerative medicine. Therefore a more complete understanding of hBMSC biology to fully exploit the cells' potential for clinical settings is important. The protein CD24 has been reported to be involved in a diverse range of processes such as cancer, adaptive immunity, inflammation, and autoimmune diseases in other cell types. Its expression in hBMSCs, which has not yet been analyzed, may add an important aspect in the understanding of hBMSC biology. The present study therefore analyzes the expression, regulation, and functional implication of the surface protein CD24 in hBMSCs. Methods used are stimulation studies with TGF beta as well as shRNA-mediated knockdown and overexpression of CD24 followed by microarray, immunocytochemistry, and flow cytometric analyses. To our knowledge, we demonstrate for the first time that the expression of CD24 is an inherent property of hBMSCs. Importantly, the data links the upregulation of CD24 to the adoption of a myofibroblast-like gene expression pattern in hBMSCs. We demonstrate that CD24 is an important modulator in transforming growth factor beta 3 (TGFβ3) signalin...Continue Reading

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Citations

Nov 26, 2019·American Journal of Physiology. Renal Physiology·Alena ParikovaOndrej Viklicky
Sep 27, 2018·Human Reproduction Update·Anna L BossJoanna L James

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Methods Mentioned

BETA
density gradient centrifugation
FACS
Fluorescence
PCR
transfection
electrophoresis
Feature Extraction
flow cytometry
confocal microscopy

Software Mentioned

Feature Extraction Software
IPA
Ingenuity Pathway Analysis
Flowing
BD FACSDiva
Prism
Feature Extraction
GraphPad
CAWOMAT

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