PMID: 7537711May 1, 1995Paper

Expression of CD44 in human hepatocellular carcinoma cell lines

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
M HaramakiM Kojiro

Abstract

CD44 is a glycosylated cell surface adhesion molecule expressed on a diverse range of cells and has several variant forms, some of which are involved in metastasis of cancer cells. Because little is known about CD44 in human hepatocellular carcinoma (HCC), we investigated its expression in tissue specimens from primary lesions (12 cases), in smear specimens from peritoneal effusions (2 cases), and in cell lines (HCC cell lines, KIM-1, KYN-1, KYN-2, KYN-3, HAK-1A, and HAK-1B; combined hepatocholangiocarcinoma cell lines, KMCH-1 and KMCH-2; and bile duct carcinoma cell lines, KMC-1 and KMBC). Immunohistochemical studies using monoclonal antibody recognizing epitope Group 1 of human CD44 molecule showed that HCC cells in all tissue specimens, including the original tumors of one smear specimen and HAK-1A, were negative for CD44; whereas, HCC cells in two-smear specimens, KIM-1, KYN-2, KYN-3, HAK-1A, HAK-1B, KMCH-1, KMC-1, and KMBC, showed positive reactions on the cell membrane. Immunostain-positive cell lines showed a positive cell rate of 51.9% to 99.8% by flow cytometric analysis. Western blotting detected CD44 protein of hemopoietic type in KIM-1, KYN-3, HAK-1A, and HAK-B and epithelial type in KMC-1 and KMBC. Southern blottin...Continue Reading

References

Jul 1, 1992·Journal of Hepatology·A IemuraM Kojiro
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Q HuJ M Vierling
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G R ScreatonJ I Bell
Feb 20, 1992·International Journal of Cancer. Journal International Du Cancer·M C KuppnerN de Tribolet
Apr 1, 1991·The Journal of Cell Biology·U H FrixenW Birchmeier
May 1, 1991·The Journal of Clinical Investigation·S JalkanenP Klemi
Jul 1, 1990·The Journal of Experimental Medicine·K MiyakeP W Kincade
Nov 15, 1990·International Journal of Cancer. Journal International Du Cancer·S ReberM Zöller
Nov 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H YanoT Nakashima
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W M GallatinW G Carter
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J P JohnsonG Riethmüller
May 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T MurakamiM Kojiro
Jun 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E A RobertsS Yang
Aug 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H YanoM Kojiro

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