Expression of CD68 in non-myeloid cell types

Scandinavian Journal of Immunology
E GottfriedM Kreutz

Abstract

CD68, the human homologue of macrosialin, is commonly regarded as a selective marker for human monocytes and macrophages. Its expression is thought to be regulated by a macrophage-specific promoter. However, several immunohistochemical studies have indicated that CD68 antibodies also react with other haematopoietic and non-haematopoietic cell types. We investigated the expression of CD68 in various primary cells and carcinoma cell lines using immunohistochemistry, flow cytometry, Western blot analysis and qRT-PCR. Weak but significant immunoreactivity was detected in lymphocytes and several tumour cell lines whereas staining of primary fibroblasts and endothelial cells was comparable to macrophages. The intensity of CD68 staining in individual cell types depended on the antibody clone and the fixation technique. Anti-CD68 mAb KP1 should be used with great caution for frozen tissue sections due to its reactivity with a wide variety of cell types. Also, care should be taken when distinguishing macrophages from fibroblasts/stromal cells in paraffin sections after formalin fixation since both cell types are stained highly positive for CD68. In accordance, mRNA expression of CD68 was not only detected in macrophages and monocytes bu...Continue Reading

References

Jun 1, 1990·Journal of Leukocyte Biology·R AndreesenG W Löhr
Aug 1, 1991·Histopathology·F FacchettiP G Grigolato
Jan 1, 1990·International Immunology·K A PulfordD Y Mason
May 1, 1988·Journal of Clinical Pathology·P M KellyJ O McGee
Jan 1, 1988·International Journal of Cancer. Supplement = Journal International Du Cancer. Supplement·U KaiserH J Radzun
Oct 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H KreipeM L Hansmann
Feb 1, 1984·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J L CordellD Y Mason
Feb 11, 1983·Journal of Immunological Methods·R AndreesenG W Löhr
Apr 1, 1993·Journal of Clinical Pathology·I A DoussisD Y Mason
Apr 1, 1995·American Journal of Clinical Pathology·A GloghiniA Carbone
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·M P RamprasadD Steinberg
Apr 5, 2003·The Journal of Biological Chemistry·Dawn O'ReillyDavid R Greaves

❮ Previous
Next ❯

Citations

May 15, 2013·Cancer Immunology, Immunotherapy : CII·Joshua M JudgeCraig L Slingluff
Jun 12, 2012·Clinical & Experimental Metastasis·Jenny A Rudnick, Charlotte Kuperwasser
Apr 11, 2013·International Journal of Clinical and Experimental Pathology·Ahmad AmanzadaFederico Moriconi
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Brian RuffellLisa M Coussens
May 16, 2013·Cardiovascular Research·Praveena S ThiagarajanMartha K Cathcart
Dec 3, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·T J E FaberM Nap
Feb 19, 2016·British Journal of Cancer·Lina OhlssonBasel Sitohy
Feb 6, 2016·Annals of the New York Academy of Sciences·Ilya ChernyavskiySuresh C Tyagi
Jan 16, 2016·Blood Cancer Journal·F BoissardL Ysebaert
Jul 27, 2010·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·K T CoschiganoJ J Kopchick
Jun 1, 2010·Microbial Pathogenesis·Juarez Antônio Simões QuaresmaMaria Irma Seixas Duarte
Oct 4, 2015·Biochimie·Giovanna LiguoriSimona Tafuri
Apr 25, 2013·Histopathology·Wei WangMichael W Beaty
Jul 4, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A FlahtA Ratajska
Dec 17, 2011·International Journal of Cancer. Journal International Du Cancer·Fu-Tsai ChungHan-Pin Kuo
May 7, 2011·American Journal of Reproductive Immunology : AJRI·Zhonghua TangSeth Guller
Nov 19, 2013·Journal of the European Academy of Dermatology and Venereology : JEADV·D TöröcsikR Adány
Jul 22, 2014·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·C E BernardUNKNOWN NIDDK Gastroparesis Clinical Research Consortium (GpCRC)
Feb 11, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yan-Hong NiYa-Yi Hou
Feb 7, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Maria DahlStefan Karlsson
Apr 11, 2015·Cancer Cell·Brian Ruffell, Lisa M Coussens
Jun 19, 2013·Current Opinion in Pharmacology·Jenny Cook, Thorsten Hagemann
Oct 15, 2013·Journal of Reproductive Immunology·Anne Cathrine StaffChristopher W G Redman
Dec 19, 2014·Molecules and Cells·Samthosh V AlahariSuresh K Alahari
Feb 9, 2016·Obesity·Adam G Goodwill
Nov 19, 2014·Antimicrobial Agents and Chemotherapy·Alvaro A OrdonezSanjay K Jain
Feb 6, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A J DemetrisD B Stolz
Jun 23, 2016·The Journal of Investigative Dermatology·Luisa SenraNicolò Costantino Brembilla
Nov 22, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Dimitry A ChistiakovYuri V Bobryshev
Mar 23, 2017·Respirology : Official Journal of the Asian Pacific Society of Respirology·Mathew S EapenSukhwinder S Sohal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Toxicology in Vitro : an International Journal Published in Association with BIBRA
S TravaglioneC Fiorentini
Proceedings of the National Academy of Sciences of the United States of America
Brian RuffellLisa M Coussens
Seminars in Thrombosis and Hemostasis
Antonella TufanoGiovanni Di Minno
© 2022 Meta ULC. All rights reserved