Expression of CD73 and A2A receptors in cells from subjects with obesity and type 2 diabetes mellitus

Immunobiology
Juan M Guzman-FloresD P Portales-Pérez

Abstract

Regulatory T cells have various mechanisms to suppress the inflammatory response, among these, the modulation of the microenvironment through adenosine and with the participation of CD39, CD73 and A2A. The aim of this study was to assess the expression of CD73 and A2A in immune cells and the effect of activation of A2A by an adenosine analogue on apoptosis in patients with obesity and type 2 diabetes mellitus (T2D). CD73 and A2A expression were analyzed by flow cytometry in lymphocyte subpopulations from patients with obesity (n = 22), T2D (n = 22), and healthy subjects (n = 20). Lymphocytes were treated with the selective A2A antagonist (ZM241385) or the selective A2A agonist (CGS21680), and apoptotic cells were detected by Annexin V. We found an increased expression of CD39 coupled to a decrease in CD73 in the patient groups with obesity and T2D compared to the control group in the different studied lymphocyte subpopulations. A2A expression was found to be increased in different subpopulations of lymphocytes from T2D patients. We also detected positive correlations between CD39+ cells and age and BMI. Meanwhile, CD73+ cells showed negative correlations with age, WHR, BMI, FPG, HbAc1, triglycerides and cholesterol. Moreover, a...Continue Reading

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Jan 1, 2016·Journal of Molecular and Cellular Cardiology·Anne LejayJames W Scholey
Jul 6, 2015·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Stefania MerighiStefania Gessi
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Dec 29, 2020·Frontiers in Pharmacology·Victor Manuel Ruiz-RodríguezDiana Patricia Portales-Pérez
Feb 23, 2021·Frontiers in Pharmacology·Anna Lisa GiulianiFrancesco Di Virgilio

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