PMID: 9168303Apr 1, 1997Paper

Expression of CD95(FAS) by gene transfer does not sensitize K562 to Fas-killing

Hematology and Cell Therapy
R MunkerM Andreeff

Abstract

CD95(FAS/Apo-1) belongs to the family of TNF receptor related molecules and is expressed on many benign and malignant types of cells. CD95 triggering is involved in the apoptotic death of lymphoid cells and may also be important in myelopoiesis. Myeloid leukemia cells are in most cases resistant to CD95 triggering despite expressing the antigen. We reasoned that myeloid leukemic cells can become sensitized to the Fas pathway of cell death if CD95 expression is restored by gene transfer. As a model, we utilized the cell line K562 which does not express CD95 on their cell surface. The gene for CD95 was introduced into K562 cells (by electroporation). As a control, the gene coding for beta-galactosidase was used. The CD95 transfected K562 cells stably expressed CD95, and had a growth pattern comparable to untransfected cells, but still remained resistant to Fas-triggering (tested using recombinant Fas-ligand). Our experiment shows that antigen expression is not a critical determinant of Fas-mediated apoptosis, but further down-stream mechanisms determine the fate of the cells. The transfected cells also had a comparable susceptibility to NK-mediated lysis which shows that the expression of CD95 does not interfere with NK-mediated ...Continue Reading

Citations

Feb 27, 2010·Progress in Neuro-psychopharmacology & Biological Psychiatry·Katja GotovacDragan Dekaris
Oct 28, 2008·European Journal of Cell Biology·Mingxia YuZhuoya Li
Mar 27, 2007·Cellular Microbiology·Claudette L FullerSina Bavari
Dec 18, 2001·International Journal of Cancer. Journal International Du Cancer·R YangA Gruber
Nov 29, 2017·Frontiers in Immunology·Milica Perišić NanutJanko Kos
Sep 5, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Yvonne J YamanakaJ Christopher Love
Sep 18, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Zahra Tayarani-NajaranSeyed Ahmad Emami
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Mar 5, 2021·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Asuka WatanabeShigetaka Shimodaira

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