Expression of cell cycle proteins in ovarian carcinoma cells in serous effusions-biological and prognostic implications

Gynecologic Oncology
B DavidsonVivi Ann Flørenes

Abstract

The aim of this study was to investigate the expression of cell cycle proteins in ovarian carcinoma cells in serous effusions and respective solid tumors. Fifty-five malignant effusions and 38 tumors (20 primary, 18 metastatic) were immunohistochemically stained for cyclin A, p27(kip1), and Ki-67. Staining extent (0-100% cells) and intensity (0-3 scale) were scored. Cyclin A and p27(kip1) expression was additionally studied in 29 malignant effusions using immunoblotting. Immunohistochemistry results in effusions were evaluated for possible association with clinicopathologic parameters. Nuclear immunoreactivity for all markers was detected on carcinoma cells in the majority of effusions using immunohistochemistry. Similarly, immunoblotting showed the presence of cyclin A and p27(kip1) in 29/29 and 25/29 specimens, respectively. Intense (3) immunoreactivity for Ki-67 was detected more often in peritoneal effusions, compared with those of pleural location (P = 0.036). Staining in primary and metastatic lesions was generally comparable to that of tumor cells in effusions. Staining for p27(kip1) was more diffuse in effusion specimens obtained prior to the institution of chemotherapy (P = 0.042). In an analysis of all effusions, an a...Continue Reading

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Citations

Jun 2, 2005·Journal of the National Cancer Institute·Mathieu BoissanMarie-Lise Lacombe
Jun 1, 2014·Cellular and Molecular Life Sciences : CMLS·Nawal BendrisBénédicte Lemmers
Jan 11, 2012·The Journal of Cell Biology·Nikola ArsicJean Marie Blanchard
Oct 6, 2007·International Journal of Cancer. Journal International Du Cancer·Yi-Fen WangLiang-Shun Wang
Jul 27, 2005·Molecular Cancer·Marci E SchanerAnne-Lise Børresen-Dale
Oct 17, 2003·Clinics in Laboratory Medicine·Ben DavidsonAasmund Berner
Sep 29, 2004·Diagnostic Cytopathology·Ben Davidson
Aug 24, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiangfeng LiLiping Xie
Apr 22, 2015·Intrinsically Disordered Proteins·Vladimir N Uversky
Jan 17, 2019·PeerJ·Sanja Radojević-ŠkodrićSnežana Pejić
Feb 10, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ben DavidsonElise C Kohn

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