PMID: 7540098Dec 1, 1994Paper

Expression of cell surface markers during differentiation of CD34+, CD38-/lo fetal and adult bone marrow cells

ImmunoMethods
J OlweusL W Terstappen

Abstract

Even though there has been considerable progress in the phenotypic characterization of CD34+ bone marrow cells, there is still limited knowledge about the cell phenotypes corresponding to functional terms such as colony-forming cells, burst-forming cells, long-term culture-initiating cells, and high-proliferative potential cells. In this study, we performed a detailed analysis of phenotypic characteristics of subsets of CD34+ cells. We compared cells from adult and fetal bone marrow to investigate whether reported functional differences are reflected in the cellular phenotypes. CD34+, CD38-/lo, HLA-DR+ cells, which have been shown to contain the most immature hematopoietic progenitor cells, stained as a homogeneous population with most monoclonal antibodies (mAbs). The antigens sLex, CD32, and CD7 were, however, heterogeneously expressed in the CD38-/lo population. Phenotypic differences in the CD34+, CD38-/lo population was found when comparing adult and fetal bone marrow cells. Adult bone marrow CD34+, CD38-/lo cells stained more brightly with CD4, Thy-1, and CD49b and more dimly with CD32 than the same population in fetal bone marrow. Certain antigens that have previously been regarded as lineage-specific were found on the C...Continue Reading

Citations

Jun 30, 1998·APMIS. Supplementum·J Olweus
Dec 1, 1995·Stem Cells·A Gee
Feb 25, 2009·Development, Growth & Differentiation·Leonardo ErminiFloriana Rosati
Nov 21, 2007·Experimental Hematology·Ian CoplandJacques Galipeau
Mar 27, 2001·Blood·D ClayM C Le Bousse-Kerdilès
Mar 12, 2017·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Yang ZhaoYong Zhao
Oct 7, 2008·Scandinavian Journal of Clinical and Laboratory Investigation·Eli TaraldsrudTorstein Egeland
Apr 30, 2017·Expert Review of Hematology·Prajwal Chaitanya Boddu, Tapan Mahendra Kadia
Jan 9, 2018·Expert Opinion on Biological Therapy·Jennifer R EvanFrancesca Bagnato

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