Expression of chemokine-like factor 1 is upregulated during T lymphocyte activation

Life Sciences
Ting LiWenling Han

Abstract

Chemokine-like factor 1 (CKLF1) is a cytokine with chemotactic effects on leukocytes and a functional ligand of CCR4. This cytokine is widely expressed and the level of expression is reported to be upregulated in asthma and rheumatoid arthritis (RA), disease conditions in which T lymphocytes are over-activated. In order to determine the expression profile of CKLF1 in activated T lymphocytes, we first employed a PCR-based method on human blood fractions cDNA panels and found that CKLF1 was upregulated in activated CD4+ and CD8+ cells, with no obvious changes in CD19+ cells. We further performed kinetic analyses of CKLF1 expression in phytohemagglutinin (PHA)-stimulated human peripheral blood lymphocytes (PBL) at both the mRNA and protein levels. In resting PBL, the constitutive expression of CKLF1 was low at mRNA level and barely detectable at the protein level; however, both were remarkably upregulated by PHA, appearing at 8h after PHA-stimulation and persisting up to 72h. These results suggest that CKLF1 may be involved in T lymphocyte activation and further study of CKLF1 function will prove valuable.

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Citations

Aug 6, 2013·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Ting LiWenling Han
Dec 29, 2009·Frontiers in Bioscience (Scholar Edition)·Zoltan SzekaneczAlisa E Koch
Feb 4, 2016·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Ke TaoHu Li
May 25, 2011·Biochemical and Biophysical Research Communications·Yan ZhangYing Wang
Apr 16, 2010·Cellular Immunology·Luning ShaoWenling Han
May 17, 2015·Protein Expression and Purification·Huihui LiuWenling Han
May 7, 2015·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Weiwei FuWenling Han
Feb 5, 2019·Molecular and Cellular Biochemistry·Pei HeFei-Yan Deng
May 14, 2019·International Immunology·Zhengyang LiuWenling Han
Sep 20, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jens EberleinDirk Homann

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