PMID: 2123350Nov 1, 1990Paper

Expression of chicken liver cell adhesion molecule fusion genes in transgenic mice

Proceedings of the National Academy of Sciences of the United States of America
M BegemannG M Edelman

Abstract

The tissue-specific expression of the chicken liver cell adhesion molecule (L-CAM) was studied by generating transgenic mice. The rat insulin II promoter was fused to a chicken L-CAM cDNA or to chicken genomic L-CAM sequences. Mice carrying the cDNA showed no expression of L-CAM. Mice carrying L-CAM genomic sequences showed expression in the beta cells of the pancreas, suggesting that sequences in introns or in flanking regions are required for expression. Murine L-CAM was undetectable in the beta cells of the pancreas of those transgenic mice expressing chicken L-CAM and thus appeared to be down-regulated, but expression of the mouse protein was not altered at other sites. Chicken L-CAM was also found in extrapancreatic tissues such as skin, kidney, liver, lung, intestine, blood vessels, and the choroid plexus and leptomeninges of the central nervous system. These findings raised the possibility that the chicken L-CAM gene contains cis regulatory elements that interfere with the specificity of a tissue-specific promoter such as the rat insulin promoter. To test this hypothesis, transgenic mice were produced with a construct containing the murine neurofilament promoter fused to genomic chicken L-CAM sequences. Chicken L-CAM was...Continue Reading

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Citations

Jan 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·G M Edelman
Oct 1, 1992·Molecular Reproduction and Development·M Cohen-TannoudjiC Babinet
Jul 1, 1992·Transgenic Research·T R MikkelsenJ P Hjorth
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Apr 29, 2009·Cellular Signalling·Maria PhilippovaTherese J Resink

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