Expression of chicken thyroid-stimulating hormone beta-subunit messenger ribonucleic acid during embryonic and neonatal development

Endocrinology
C C GregoryTom E Porter

Abstract

The importance of thyroid hormone from embryonic through neonatal life has been documented in both avian and mammalian species. However, the regulation of thyroid hormone production during this period is not completely understood. The objective of this study was to characterize expression of chicken TSHbeta messenger RNA (mRNA) compared with that of thyroid hormones and GH in embryonic and neonatal chickens. Total pituitary RNA was extracted on embryonic days (e-) 11, 13, 15, 17, and 19 and neonatal days (d-) 1, 3, 6, 9, and 12 and subjected to ribonuclease protection assays (RPA) for chicken TSHbeta mRNA. TSHbeta mRNA levels increased through e-19, with e-19 levels being greater than those at all other embryonic ages (P < 0.05). Levels decreased markedly on d-1, then slowly increased to d-6 and stayed elevated through d-12. RIAs were performed for T4, T3, and GH at the same ages. Serum T4 levels increased slowly from less than 1.0 ng/ml on e-11 to a peak of 6.6 ng/ml on d-1 (P < 0.05). After the peak on d-1, posthatch T4 levels stabilized between 3.5-4.5 ng/ml through d-12 (P < 0.05). T3 concentrations were less than 0.25 ng/ml on e-11, increased dramatically between e-19 and d-1 (P < 0.05), and remained high throughout the re...Continue Reading

References

Nov 1, 1979·General and Comparative Endocrinology·S HarveyA Chadwick
Aug 1, 1977·General and Comparative Endocrinology·R C Thommes, V W Hylka
Aug 1, 1976·General and Comparative Endocrinology·D B King, C R King
Jul 1, 1987·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·E R KühnE Decuypere
Dec 1, 1973·General and Comparative Endocrinology·D B King, C R King
Dec 1, 1984·The Journal of Experimental Zoology·D B King, J D May
Sep 1, 1982·Early Human Development·A H KleinD A Fisher
Jun 1, 1982·Poultry Science·E DecuypereH Michels
Aug 1, 1997·General and Comparative Endocrinology·C C Gregory, T E Porter

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Citations

Dec 21, 2010·General and Comparative Endocrinology·Laura E EllestadTom E Porter
Aug 30, 2006·General and Comparative Endocrinology·F M Anne McNabb
Feb 7, 2006·General and Comparative Endocrinology·F M Anne McNabb
Nov 16, 2005·Proteomics·David AgudoRafael Linares

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