Expression of chitinase genes of Metarhizium anisopliae isolates in lepidopteran pests and on synthetic media

Journal of Basic Microbiology
G V S Bhanu PrakashP B Kirti

Abstract

Pathogenecity of the well characterized entomopathogenic fungus Metarhizium anisopliae used for biocontrol of a wide range of insect pests secretes hydrolytic enzymes that degrade the host cuticle. The chitinolytic activity of high and low virulent isolates of M. anisopliae was assayed on minimal medium (MM) + colloidal chitin and MM supplemented with insect cuticles. Ex- pression pattern of four chitinase genes (chitinase (chi), chi 1, chi 2, chi 3) was profiled during pathogenic stages of the entomopathogen under in vitro and in vivo conditions. Reverse-transcription polymerase chain reaction (RT-PCR) analysis confirmed that chitinase cDNAs were expressed during the germination of fungus under nutrient-deprived conditions. RT-PCR analysis performed for the four chitinase genes on the two insect hosts Spodoptera litura and Helicoverpa armigera at six developmental stages of the pathogen displayed up-regulation in S. litura at mycosed and conidiated condition while with H. armigera there was expression only after 48 h of incubation. Differential expression of chi, chi 1 and chi 2 genes in vitro (nitrogen rich and nitrogen limiting media) and in vivo (live insect hosts S. litura and H. armigera) implicate the role of substrate d...Continue Reading

References

Jan 22, 2004·Canadian Journal of Microbiology·César Milton BarattoAugusto Schrank
Apr 2, 2004·Current Microbiology·Cristine Chaves BarretoMarilene Henning Vainstein
Jan 11, 2005·Applied and Environmental Microbiology·Weiguo FangYan Pei
Jun 9, 2006·Mycopathologia·Li Duo-Chuan
Apr 22, 2010·Gene·Juliano Tomazzoni BoldoAugusto Schrank

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