Expression of cholesteryl ester transfer protein (CETP) in germinal centre B cells and their neoplastic counterparts

Histopathology
K ItoS Mori

Abstract

Cholesteryl ester transfer protein (CETP) is known to facilitate the transfer of lipids between plasma lipoproteins. Previous studies on human tissues have determined that the spleen contains large amounts of CETP mRNA, while the exact location of CETP in such organs remains unknown. In the present study, our aim was to locate CETP protein expression at the cellular level in human normal and neoplastic lymphoid organs. In-situ hybridization (ISH) and immunohistochemistry were applied to pathology specimens. A specific rabbit anti-CETP antibody was used for immunohistochemical analysis, together with another CETP-specific monoclonal antibody. A riboprobe for ISH was derived from CETP cDNA. Immunohistochemically, CETP was localized in germinal centre B cells and a proportion of marginal zone B cells. ISH showed that CETP mRNA was located mostly in the same areas. When 141 malignant lymphomas of various subtypes were studied, high expression of CETP, equivalent to that found in normal germinal centre B cells, was demonstrated in lymphoma subtypes that are currently regarded as the neoplastic counterparts of primarily germinal centre B cells. CETP localizes B cells in germinal centres, a proportion of post-germinal centre B cells a...Continue Reading

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Citations

Apr 16, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Guojun ZhangZhuogang Liu
Aug 26, 2009·Molecular Cancer·Ponniah SelvakumarRajendra K Sharma
Apr 11, 2021·Kidney International·Vanderlene L KungEdmund Huang

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