Expression of clock-related genes in benign and malignant adrenal tumors.

Endocrine
Anna AngelousiEva Kassi

Abstract

Although the effect of the central clock system on adrenal function has been extensively studied, the role of the peripheral clock system in adrenal tumorigenesis remains largely unexplored. In this study we investigated the expression of clock-related genes in normal adrenocortical tissue and adrenocortical tumors. Twenty-seven fresh frozen human adrenal tissues including 13 cortisol secreting adenomas (CSA), seven aldosterone producing adenomas (APA), and seven adrenocortical carcinomas (ACC) were collected. CLOCK, BMAL1, PER1, CRY1, Rev-ERB, and RORα mRNA and protein expression were determined by qPCR and immunoblotting in pathological tissues and compared with the adjacent normal adrenal tissues. A significant downregulation of PER1, CRY1, and Rev-ERB compared with their normal tissue was demonstrated in CSA. All clock-related genes were overexpressed in APA compared with their normal tissue, albeit not significantly. A significant upregulation of CRY1 and PER1 and downregulation of BMAL1, RORα, and Rev-ERB compared with normal adrenal tissue was observed in ACC. BMAL1 and PER1 were significantly downregulated in APA compared with CSA. CLOCK, CRY1, and PER1 were upregulated, whereas BMAL1, RORα, and Rev-ERB were downregulat...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 1, 1964·Comparative Biochemistry and Physiology·R V ANDREWS, G E FOLK
Nov 8, 2005·Cell Metabolism·Atsushi IshidaHitoshi Okamura
Dec 17, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yvonne M Ulrich-LaiWilliam C Engeland
Jan 3, 2006·Endocrine·William C Engeland, Michelle M Arnhold
Jun 16, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hongnian GuoEric L Bittman
Sep 22, 2006·Human Molecular Genetics·Caroline H Ko, Joseph S Takahashi
Mar 1, 2007·Physiological Genomics·Damian G RomeroCelso E Gomez-Sanchez
Nov 26, 2009·Nature Reviews. Cancer·Saurabh Sahar, Paolo Sassone-Corsi
Jan 29, 2010·Trends in Endocrinology and Metabolism : TEM·Nancy NaderTomoshige Kino
Feb 19, 2011·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·C CampinoM Seron-Ferre
Apr 19, 2012·International Journal of Cancer. Journal International Du Cancer·Momen ElshazleyYoshinori Hasegawa
May 29, 2013·The International Journal of Biological Markers·Theodoros KarantanosDimitrios Pektasides
Apr 1, 2012·Hormones : International Journal of Endocrinology and Metabolism·Eva N Kassi, George P Chrousos
Oct 25, 2013·American Journal of Physiology. Renal Physiology·Jacob RichardsMichelle L Gumz
Oct 11, 2014·Biochemistry·Aziz SancarNuri Ozturk
Sep 4, 2015·Diabetes, Obesity & Metabolism·M PerelisJ Bass
Apr 21, 2017·International Journal of Molecular Sciences·Anton Shostak
May 16, 2017·Frontiers in Endocrinology·Nicolas C NicolaidesGeorge P Chrousos
Nov 24, 2017·Future Oncology·Silke Kiessling, Nicolas Cermakian
Feb 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sam-Moon KimDavid J Earnest
Jun 8, 2018·International Journal of Molecular Sciences·Martina TettiSilvia Monticone
Sep 13, 2018·Scientific Reports·Aurore Woller, Didier Gonze
Apr 9, 2019·Endocrine-related Cancer·Anna AngelousiGeorge P Chrousos

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