PMID: 2500148Jul 7, 1989Paper

Expression of cloned Saccharomyces diastaticus glucoamylase under natural and inducible promoters

Biochimica Et Biophysica Acta
M VanoniL Alberghina

Abstract

Any one of three homologous genes - STA1, STA2 and STA3 - encoding glucoamylase isozymes I, II and III respectively, allows the Saccharomyces species to utilize starch as a sole carbon source. We show in this paper that glucoamylase II production can be increased 4-fold over the level produced by STA2 strains, by using a two-step fermentation and a yeast strain transformed with a high-copy-number plasmid carrying the STA2 gene. The accumulation of anomalous STA2 mRNA species, mainly differing at their 5' ends, and saturation of step(s) in the secretory pathway appear to be among the major factors limiting glucoamylase expression in synthetic media.

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Citations

Feb 1, 1991·Applied Microbiology and Biotechnology·D PorroL Alberghina
Sep 23, 2000·Journal of Microbiological Methods·D PorroM Vanoni
Apr 24, 2010·Applied Biochemistry and Biotechnology·Fabiana Carina PavezziRoberto da Silva
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·I S PretoriusJ Marmur
Aug 3, 2014·Eukaryotic Cell·Jürgen Wendland

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