Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary

Biological Research
Pengfei LiLihua Lyu

Abstract

Cocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, such as regulation of food intake, energy homeostasis, and reproduction. The hens egg-laying rate mainly depends on the developmental status of follicles, expression of CART have not been identified from hen follicles, the regulatory mechanisms of CART biological activities are still unknown. The objective of this study was to characterize the mRNA expression of CART in hen follicular granulosa cells and determine CART peptide localization and regulatory role during follicular development. Small white follicles (1-2 mm in diameter) were treated for RNA isolation; Small white follicles (1-2 mm in diameter) and large white follicles (4-6 mm in diameter) were treated for immunohistochemical localization and large white follicles (4-6 mm in diameter), small yellow follicles (6-8 mm in diameter), large yellow follicles (9-12 mm in diameter), mature follicles (F5, F4, F3, F2, F1, >12 mm in diameter) were treated for RNA isolation and Real time PCR. The results s...Continue Reading

References

Jan 1, 1988·Journal of Reproduction and Fertility·S K GuptaM A Walker
Dec 1, 1996·Biology of Reproduction·O J GintherK Kot
Jun 24, 2003·Animal Reproduction Science·A L Johnson
Nov 26, 2008·Animal Reproduction Science·Okanlawon OnagbesanEddy Decuypere
Dec 9, 2008·General and Comparative Endocrinology·A L Johnson, Dori C Woods

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SPASS
DNAMAN
Primer premier

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