Abstract
The collagenase activity and the fpcol gene were examined in Flavobacterium psychrophilum isolates from cold-water disease (CWD)-affected ayu, Plecoglossus altivelis. Collagenase expression was closely related to the accumulated mortality of CWD-affected ayu. RT-qPCR and bacterial challenge experiments showed that F. psychrophilum ayu isolate WA-1 expressed the fpcol gene more actively and was more virulent than ayu isolate WA-2. The amago (Oncorhynchus masou) isolate WB-1, which possesses a pseudo-fpcol gene, was not harmful to ayu. Hitherto, the well-studied metalloproteases Fpp1 and Fpp2 have been considered virulence factors. However, the most virulent isolate against ayu (WA-1) showed no Fpp activity because of a deletion mutation or an insertion of a transposon in the fpp genes. The less virulent WA-2 isolate showed only Fpp1 activity. Taken together, these results suggest that collagenolytic activity, but not Fpp activity, is related to the virulence of F. psychrophilum isolates in CWD-affected ayu.
References
Jan 11, 1991·Nucleic Acids Research·I V BoniN V Tzareva
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Nov 1, 1993·Bioscience, Biotechnology, and Biochemistry·Y SasagawaK Izaki
Nov 1, 1995·Bioscience, Biotechnology, and Biochemistry·Y SasagawaY Kamio
Apr 27, 2001·European Journal of Biochemistry·L L MacLeanW W Kay
May 26, 2001·Applied and Environmental Microbiology·P SecadesJ A Guijarro
Apr 12, 2002·General and Comparative Endocrinology·Wen-Hsiung ChenChing-Fong Chang
Oct 14, 2003·FEMS Microbiology Letters·P SecadesJ A Guijarro
Dec 12, 2003·Diseases of Aquatic Organisms·Shotaro IzumiHisatsugu Wakabayashi
Jan 3, 2006·Diseases of Aquatic Organisms·Satoshi Miwa, Chihaya Nakayasu
Jun 6, 2006·Applied and Environmental Microbiology·B AlvarezJ A Guijarro
Jun 16, 2006·RNA·Maxim V SukhodoletsSankar Adhya
Nov 11, 2006·Vaccine·Masatoshi AokiSyun-Ichirou Oshima
Apr 11, 2007·Fish & Shellfish Immunology·Benjamin R LaFrentzKenneth D Cain
Apr 12, 2007·Diseases of Aquatic Organisms·Ponnerassery S SudheeshKenneth D Cain
Jun 27, 2007·Nature Biotechnology·Eric DuchaudAbdenour Benmansour
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Keiko SatoKoji Nakayama
Dec 30, 2009·Journal of Bacteriology·Ryan G RhodesMark J McBride
Jan 11, 2011·Applied and Environmental Microbiology·Shohreh HesamiJanet I Macinnes
Apr 26, 2011·Journal of Bacteriology·Naoko TeramuraShinkichi Irie
Jul 26, 2011·Journal of Bacteriology·Ryan G RhodesMark J McBride
Sep 20, 2011·Applied and Environmental Microbiology·Marie TouchonEric Duchaud
May 15, 2012·Journal of Bacteriology·Abhishek ShrivastavaMark J McBride
Nov 6, 2012·Journal of Bacteriology·Mark J McBride, Yongtao Zhu
Apr 16, 2013·Journal of Bacteriology·Nozomu ObanaKouji Nakamura
Citations
Dec 10, 2016·Bioscience, Biotechnology, and Biochemistry·Jun QianKiyoshi Yasukawa
Nov 23, 2017·Frontiers in Microbiology·David Pérez-PascualEric Duchaud
Dec 19, 2017·Frontiers in Microbiology·Shicheng ChenEdward D Walker
Jan 13, 2019·Applied and Environmental Microbiology·T RochatE Duchaud
Jan 5, 2017·Bioscience, Biotechnology, and Biochemistry·Hitoshi NakayamaKenichi Amano
Oct 27, 2016·Microbial Ecology·Reetta PenttinenLotta-Riina Sundberg
Jun 11, 2020·PeerJ·Lubov ChernogorSergei Belikov
Nov 5, 2020·International Journal of Molecular Sciences·Ivan PetrushinLubov Chernogor
Aug 28, 2021·Microorganisms·Daniel CastilloMathias Middelboe