Expression of colonization factor CS5 of enterotoxigenic Escherichia coli (ETEC) is enhanced in vivo and by the bile component Na glycocholate hydrate.

PloS One
Matilda NicklassonAnn-Mari Svennerholm

Abstract

Enterotoxigenic Escherichia coli (ETEC) is an important cause of acute watery diarrhoea in developing countries. Colonization factors (CFs) on the bacterial surface mediate adhesion to the small intestinal epithelium. Two of the most common CFs worldwide are coli surface antigens 5 and 6 (CS5, CS6). In this study we investigated the expression of CS5 and CS6 in vivo, and the effects of bile and sodium bicarbonate, present in the human gut, on the expression of CS5. Five CS5+CS6 ETEC isolates from adult Bangladeshi patients with acute diarrhoea were studied. The level of transcription from the CS5 operon was approximately 100-fold higher than from the CS6 operon in ETEC bacteria recovered directly from diarrhoeal stool without sub-culturing (in vivo). The glyco-conjugated primary bile salt sodium glycocholate hydrate (NaGCH) induced phenotypic expression of CS5 in a dose-dependent manner and caused a 100-fold up-regulation of CS5 mRNA levels; this is the first description of NaGCH as an enteropathogenic virulence inducer. The relative transcription levels from the CS5 and CS6 operons in the presence of bile or NaGCH in vitro were similar to those in vivo. Another bile salt, sodium deoxycholate (NaDC), previously reported to indu...Continue Reading

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Citations

Dec 30, 2014·Expert Review of Vaccines·Åsa SjölingAnn-Mari Svennerholm
Nov 3, 2015·Environmental Microbiology·Lucia Gonzales-Siles, Åsa Sjöling
May 10, 2017·Critical Reviews in Microbiology·Elio RossiUte Römling
Nov 15, 2019·MSphere·Rachael B ChaninChristina S Faherty
Aug 22, 2018·Proceedings of the National Academy of Sciences of the United States of America·Alexander A CroftsM Stephen Trent
Jul 29, 2016·Clinical Microbiology Reviews·Jeticia R SistrunkChristina S Faherty

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Datasets Mentioned

BETA
AJ224079
UO4846
U014639.1

Methods Mentioned

BETA
ELISA
PCR
electrophoresis

Software Mentioned

GraphPad
GraphPad Prism
Primer Express

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