Expression of components of the insulin-like growth factor system and gonadotropin receptors in bovine cumulus-oocyte complexes during oocyte maturation

Domestic Animal Endocrinology
F NuttinckY Heyman

Abstract

IGF system expression has been largely explored in the bovine follicular wall whereas it remains poorly studied in the COC. Using semi-quantitative RT-PCR and Western blot analysis, we have investigated spatial and temporal expression of IGF-1, IGFR-1, IGFBP-2, IGFBP-4, as well as gonadotropin receptors in bovine COC during oocyte maturation. In addition, we have compared changes in the IGF system and FSHR expression during in vitro maturation in TCM199 alone or in the presence of 10 ng/ml of EGF. The transcripts for IGFR-1 and IGFBP-2 were detected in cumulus and germinal cells whereas IGF-1, IGFBP-4 and FSHR mRNA were restricted to cumulus cells. Topography of the IGF system and gonadotropin receptor expression within COC were unaffected by the maturation step. In contrast, levels of IGFBP-2 and FSHR expression decreased (P < 0.05) in matured COC. Under defined culture conditions, IGFBP-2 and FSHR mRNA expression remained at a high level in TCM199 alone and were significantly reduced (P < 0.05) in the presence of 10 ng/ml EGF after a 24 h period of in vitro maturation. In conclusion, our results demonstrate a cell-specific pattern of IGF system member gene expression within bovine COC suggesting interaction between the somati...Continue Reading

References

Jan 11, 1991·Advances in Experimental Medicine and Biology·H WernerD LeRoith
Jan 1, 1989·Reproduction, Nutrition, Development·B Marquant-Le GuienneC Thibault
Feb 1, 1995·Endocrine Reviews·J I Jones, D R Clemmons
Jan 1, 1995·Progress in Growth Factor Research·D R ClemmonsD K Ankrapp
Jan 1, 1996·Hormone Research·P MongetD Monniaux
Mar 10, 1998·Molecular Reproduction and Development·J J EppigK Wigglesworth
Apr 6, 2000·The Journal of Endocrinology·D G ArmstrongR Webb
Jan 5, 2002·Molecular Reproduction and Development·F NuttinckG Charpigny
Jun 8, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·D G ArmstrongK J Woad
Jul 27, 2002·Domestic Animal Endocrinology·Philippe MongetDanielle Monniaux

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Citations

Oct 28, 2005·Reproductive Biology and Endocrinology : RB&E·Kanako KumamotoTakato Terada
Sep 8, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Elizabeth M MackJennifer R Wood
Nov 26, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·M A VelazquezH Niemann
Sep 5, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Marcos H BarretaPaulo Bayard D Gonçalves
Jun 8, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·L A RispoliJ L Edwards
Apr 30, 2014·Animal : an International Journal of Animal Bioscience·J DupontM Reverchon
Aug 16, 2008·Domestic Animal Endocrinology·M A VelazquezD C Wathes
Mar 21, 2007·Anatomia, histologia, embryologia·H H OrtegaE J Gimeno
Nov 15, 2006·Animal Reproduction Science·C E FarinS Kennedy-Stoskopf
Jul 2, 2005·Animal Reproduction Science·F SchneiderW Kanitz
Mar 19, 2013·Health Research Policy and Systems·David R Walwyn
Jul 13, 2007·Biology of Reproduction·Nicole AcevedoGary D Smith
Sep 14, 2016·Reproduction, Fertility, and Development·Rafaela S LimaFabíola F Paula-Lopes
Jun 4, 2019·Reproduction in Domestic Animals = Zuchthygiene·Leda Maria Costa PereiraNucharin Songsasen
May 29, 2020·Tropical Animal Health and Production·Paulo Roberto AdonaMoysés Dos Santos Miranda
Nov 19, 2019·Molecular Reproduction and Development·Ahmed GadRadek Prochazka
Jan 1, 2018·Reproduction, Fertility, and Development·Lex Bach
Jan 17, 2013·American Journal of Physiology. Endocrinology and Metabolism·Sylvain AuclairSvetlana Uzbekova
Dec 10, 2020·Animals : an Open Access Journal From MDPI·Ahmed GadRadek Procházka
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Lorena Fernandez-GonzalezKatarina Jewgenow
Sep 27, 2013·Journal of Ovarian Research·Mourad AssidiMarc-André Sirard

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