Expression of constitutively active angiotensin receptors in the rostral ventrolateral medulla increases blood pressure

Hypertension
A M AllenWalter G Thomas

Abstract

Angiotensin type 1A (AT(1A)) receptors are expressed within the rostral ventrolateral medulla, and microinjections of angiotensin II into this region increase sympathetic vasomotor tone. To determine the effect of sustained increases in AT(1A) receptor density or activity in rostral ventrolateral medulla, we used radiotelemetry to monitor blood pressure in conscious rats before and after bilateral microinjection into the rostral ventrolateral medulla of adenoviruses encoding the wild-type AT(1A) receptor or a constitutively active version of the receptor (Asn111Gly, [N111G]AT(1A)). The constitutively active receptor signals in the absence of angiotensin II. Adenovirus-directed receptor expression was extensively characterized both in vitro and in vivo. We established that adenoviral infection was limited to the rostral ventrolateral medulla and that receptor expression was sustained for > or =10 days; we also observed that adenoviral transgene expression occurs in glia, with no transgene expression observed in neurons of the rostral ventrolateral medulla. Rats receiving the wild-type AT(1A) receptor showed no change in blood pressure, whereas animals receiving the [N111G]AT(1A) receptor displayed an increase in blood pressure t...Continue Reading

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Citations

Sep 25, 2009·Cellular and Molecular Neurobiology·L H LinW T Talman
Sep 4, 2008·Current Hypertension Reports·Scott H Carlson, J Michael Wyss
Apr 28, 2007·Nature Methods·Sergey Kasparov
Jul 15, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Huiping LiCurt D Sigmund
Aug 8, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Gang WangJoseph P Pierce
Apr 4, 2007·The Journal of Clinical Investigation·Koji SakaiCurt D Sigmund
Jan 15, 2013·International Journal of Hypertension·Wencheng LiYumei Feng
Jan 8, 2010·Autonomic Neuroscience : Basic & Clinical·Valdir A Braga
Sep 3, 2009·Pharmacology & Therapeutics·Adolfo E CuadraMohan K Raizada
Dec 9, 2008·Molecular and Cellular Endocrinology·Christophe Petrel, Eric Clauser
Jul 30, 2011·Journal of Neurochemistry·E L O'CallaghanA M Allen
Sep 8, 2011·Journal of Veterinary Science·Valdir A Braga
May 27, 2010·Journal of Hypertension·Alain G Dupont, Sofie Brouwers
Sep 14, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Kengo IsegawaKenji Sunagawa
May 7, 2020·American Journal of Hypertension·Yoshitaka Hirooka
Apr 7, 2018·Frontiers in Molecular Neuroscience·Agnese SecondoDiana Amantea

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