Expression of constructs of the neuronal isoform of myosin-Va interferes with the distribution of melanosomes and other vesicles in melanoma cells

Cell Motility and the Cytoskeleton
João Carlos da Silva BizarioEnilza Maria Espreafico

Abstract

Myosin-Va has been implicated in melanosome translocation, but the exact molecular mechanisms underlying this function are not known. In the dilute, S91 melanoma cells, melanosomes move to the cell periphery but do not accumulate in the tips of dendrites as occurs in wild-type B16 melanocytes; rather, they return and accumulate primarily at the pericentrosomal region in a microtubule-dependent manner. Expression of the full-length neuronal isoform of myosin-Va in S91 cells causes melanosomes to disperse, occupying a cellular area approximately twice that observed in non-transfected cells, suggesting a partial rescue of the dilute phenotype. Overexpression of the full tail domain in S91 cells is not sufficient to induce melanosome dispersion, rather it causes melanosomal clumping. Overexpression of the head and head-neck domains of myosin-Va in B16 cells does not alter the melanosome distribution. However, overexpression of the full tail domain in these cells induces melanosome aggregation and the appearance of tail-associated, aggregated particles or vesicular structures that exhibit variable degrees of staining for melanosomal and Golgi beta-COP markers, as well as colocalization with the endogenous myosin-Va. Altogether, the ...Continue Reading

References

Dec 1, 1985·Molecular and Cellular Biology·G I EvanJ M Bishop
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·D W ProvanceJ A Mercer
Aug 15, 1997·The Journal of Biological Chemistry·S E BenashskiS M King
Dec 31, 1997·Molecular Biology of the Cell·A A NascimentoE M Espreafico
Apr 16, 1998·Current Biology : CB·S L Rogers, V I Gelfand
Apr 16, 1998·Current Biology : CB·V I RodionovG G Borisy
Jun 23, 1998·Current Biology : CB·J F Kelleher, M A Titus
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·E M EspreaficoB Kachar
Jul 25, 1998·Cell Motility and the Cytoskeleton·X WuJ A Hammer
Nov 25, 1998·Biochemical and Biophysical Research Communications·J LambertL Messiaen
Apr 10, 1999·Molecular Biology of the Cell·S L Reck-PetersonM S Mooseker
Jul 3, 1999·The Journal of Biological Chemistry·A E El-Husseini, S R Vincent
Nov 30, 1999·Pigment Cell Research·M C Tuma, V I Gelfand
Feb 26, 2000·The Journal of Investigative Dermatology·M HaraB A Gilchrest
Apr 20, 2000·The Journal of Investigative Dermatology·H R ByersB A Gilchrest
Feb 13, 2001·Biochemical and Biophysical Research Communications·A OhyamaM Igarashi
Mar 27, 2001·The Journal of Cell Biology·A N HumeM C Seabra
Apr 11, 2001·Molecular Biology of the Cell·J S BergR E Cheney
Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·L E MatesicN A Jenkins
Aug 18, 2001·Current Biology : CB·D P MulvihillJ S Hyams
Aug 18, 2001·Science·R E Cheney, O C Rodriguez
Aug 23, 2001·The Journal of Biological Chemistry·S B TauhataR E Larson
Sep 8, 2001·Science·R G Webster

❮ Previous
Next ❯

Citations

Feb 3, 2005·The Journal of Cell Biology·Natasha PashkovaLois S Weisman
Feb 27, 2003·The Journal of Investigative Dermatology·Wendy WestbroekJean Marie Naeyaert
Jan 6, 2004·Journal of Neurobiology·Paul C Bridgman
Mar 12, 2008·Cell Motility and the Cytoskeleton·Maria Cristina S PrancheviciusRoy E Larson
Mar 23, 2010·BMC Immunology·Justin JohnHardev Pandha

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.