Expression of Cre recombinase in retinal Müller cells.

Vision Research
Yumi UekiYun-Zheng Le

Abstract

In an effort to generate inducible RPE-specific Cre mice using a 3.0-kb human vitelliform macular dystrophy-2 (VMD2) promoter, we identified a mouse line with unanticipated Cre activity in the neural retina, including Müller glial cells. Müller cells play important roles in the function and maintenance of the retina, and this mouse line would be potentially useful for conditional gene targeting in Müller glia. We therefore characterized the timing, inducibility, and cell specificity of Cre expression, as well as Müller cell-specific efficiency of Cre-mediated recombination in this mouse line. Transgenic mice carrying cassettes of human P(VMD2)-rtTA and TRE-cre were generated. Cre expression was characterized using a Cre-activatable lacZ reporter mouse line (R26R) and a floxed interleukin six signal transducing receptor (gp130) mouse line. beta-Galactosidase (beta-gal) assay and immunohistochemical analysis of VMD2-cre/R26R double transgenic mice indicated that Cre activity was detected in cells located in the inner nuclear layer, with prominent expression of beta-gal in Müller cells. Cre activity was also detected in photoreceptors in the outer nuclear layer. PCR analysis demonstrated that Cre-mediated recombination initiated b...Continue Reading

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Citations

Dec 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yumi UekiJohn D Ash
Sep 22, 2009·The Journal of Pathology·Yanyan BaiYun-Zheng Le
Jan 14, 2010·Journal of Neurochemistry·Yun-Zheng LeLixin Zheng
Dec 3, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xue CaiMuna I Naash
Oct 26, 2018·Investigative Ophthalmology & Visual Science·Christine A Curcio
Oct 22, 2020·Journal of Lipid Research·Sriganesh Ramachandra Rao, Steven J Fliesler
Mar 5, 2021·Journal of Lipid Research·Sriganesh Ramachandra Rao, Steven J Fliesler
Dec 3, 2020·Experimental Eye Research·Ming ChenDouglas Vollrath
Aug 15, 2021·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M N Perry, C L Smith

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