Expression of cryptantigen Th on paroxysmal nocturnal hemoglobinuria erythrocytes in association with a hemolytic exacerbation

The Journal of Clinical Investigation
H NakakumaK Takatsuki

Abstract

Paroxysmal nocturnal hemoglobinuria (PNH) erythrocytes lack complement regulatory membrane proteins and are susceptible to complement. Although the critical role of complement in intravascular hemolysis in PNH is accepted, the precise mechanism of complement activation in vivo is unknown. Accordingly, in a PNH patient who was suffering from a hemolytic precipitation soon after a common cold-like upper respiratory infection, we analyzed the erythrocytes with lectins and by flow cytometry to detect membrane alteration that lead to complement activation. The lectin reactivity of erythrocytes showed the expression of cryptantigen Th. The patient serum at the time of the hemolysis induced the expression of Th on erythrocytes from PNH patients and from healthy volunteers in vitro, whereas neither the patient serum after recovery from the hemolysis nor blood type-matched control serum from healthy donor showed this activity. Moreover, autologous serum selectively hemolyzed Th+ PNH erythrocytes, but not Th- PNH erythrocytes, or Th+ control erythrocytes. Hemolysis was not observed either in complement-inactivated serum or in blood type-matched cord blood serum, which lacks natural antibodies to cryptantigens. These findings indicate tha...Continue Reading

References

Jun 3, 1978·Lancet·G W BirdA Pollock
Dec 1, 1991·The Journal of Experimental Medicine·K HorikawaK Takatsuki
Jan 1, 1990·Pediatric Pathology·R W Novak
May 1, 1990·The Journal of Clinical Investigation·H NakakumaK Takatsuki
Sep 1, 1988·Transfusion Medicine Reviews·C LeveneN Manny
May 1, 1987·Transfusion·J H HermanP M Ness
Feb 1, 1987·British Journal of Haematology·J H HermanP M Ness
Apr 1, 1984·The Journal of Clinical Investigation·C J ParkerW F Rosse
May 1, 1984·Transfusion·Y OkuboH Yamaguchi
Jan 27, 1994·The New England Journal of Medicine·T MiyataT Kinoshita

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Citations

Nov 1, 1996·Critical Reviews in Oncology/hematology·H Nakakuma, T Kawaguchi
Aug 8, 1998·British Journal of Haematology·M IwanagaM Tomonaga
Aug 7, 2007·International Journal of Hematology·Tatsuya Kawaguchi, Hideki Nakakuma
Mar 12, 2003·International Journal of Hematology·Hideki Nakakuma, Tatsuya Kawaguchi
Mar 12, 2003·International Journal of Hematology·Wendell F Rosse, Junichi Nishimura
Dec 26, 2007·Annals of Hematology·Georgia KaiafaApostolos Hatzitolios
Mar 28, 2006·Transfusion Medicine Reviews·Lawrence D Petz
Mar 31, 2015·Revista brasileira de hematologia e hemoterapia·Ana Paula de AzambujaRicardo Pasquini
Sep 1, 1996·American Journal of Hematology·H Nakakuma
Sep 27, 2000·Anesthesia and Analgesia·S KathirvelP Sujatha

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