Expression of Cryptosporidium parvum thioredoxin peroxidase in COS-7 cells confers radioprotection

Experimental Parasitology
Semie HongJae-Ran Yu

Abstract

Cryptosporidium parvum is one of the most radioresistant organisms identified to date. In a previous study, we found that thioredoxin peroxidase (CpTPx) was significantly upregulated in this species following exposure to high dose (10 kGy) of γ-irradiation. To assess the potential of CpTPx to confer radioprotection in mammalian cells, it was expressed in COS-7 African green monkey kidney cells (CpTPx-COS7). For comparison, the thioredoxin peroxidase of Cryptosporidium muris (CmTPx) was also expressed in these cells (CmTPx-COS7 cells), which has been confirmed to have lesser antioxidant activity than CpTPx in the previous study. Notably, the survival rates of CpTPx-COS7 cells were significantly higher (12-22%) at 72 h after 8 Gy irradiation than CmTPx-COS7 or non-transfected COS-7 (ntCOS-7) counterparts. In addition, CpTPx revealed a 50% of ROS reduction in irradiated CpTPx-COS7 cells, while γ-H2AX DNA damage marker expression was not significantly changed. Furthermore, the amount of apoptosis only increased to about 120% after 2-8 Gy irradiation compared to 200-300% increase observed in ntCOS-7 cells. CmTPx was shown to have antioxidant and DNA damage protection activities; however, these activities were always lower than those...Continue Reading

References

Nov 1, 1995·International Journal of Radiation Oncology, Biology, Physics·W C DeweyR E Meyn
Apr 16, 1998·The Journal of Biological Chemistry·E P RogakouW M Bonner
Jun 25, 2002·Journal of Molecular Biology·Curt A DaveyTimothy J Richmond
Aug 23, 2002·Microbes and Infection·Saul Tzipori, Honorine Ward
Oct 3, 2002·Seminars in Liver Disease·Xian-Ming Chen, Nicholas F LaRusso
Jan 9, 2003·Trends in Biochemical Sciences·Zachary A WoodLeslie B Poole
Jul 26, 2003·The Journal of Parasitology·J R Yu, W Y Park
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jeffrey FillinghamNevan J Krogan
Oct 17, 2006·Parasitology Today·S McGonigleE R James
Jan 26, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Malgorzata KajsturaZbigniew Darzynkiewicz
Jun 5, 2007·Nature Protocols·David ShechterSandra B Hake
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Jun 9, 2009·Cancer Letters·Bo ZhangYongping Su
Jun 27, 2009·Parasitology Research·M ShahiduzzamanA Daugschies
Jul 6, 2010·Experimental Parasitology·Soo-Ung LeeJae-Ran Yu
Sep 3, 2010·Journal of Nucleic Acids·Monika PodhoreckaPrzemyslaw Bozko
Oct 18, 2011·Experimental Parasitology·Migyo JoungJae-Ran Yu

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Citations

Oct 31, 2017·Frontiers in Immunology·Shalini ChakrabortyParesh Sharma
Feb 27, 2021·International Journal of Biological Macromolecules·Pankaj Kumar ChaturvediWoo-Yoon Park

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