Expression of cspH upon nutrient up-shift in Salmonella enterica serovar Typhimurium

Archives of Microbiology
B H KimYong Keun Park

Abstract

The gene cspH, which encodes one of the cold-shock proteins in Salmonella enterica serovar Typhimurium, has previously been reported to be induced during early exponential phase at 37 degrees C. In the present study, the expression of cspH upon nutrient up-shift at 37 degrees C was investigated and found to be affected by DNA gyrase and DNA-binding protein Fis. When cells at stationary phase were subcultured into a rich medium, the mRNA level of cspH increased dramatically prior to the first cell division. However, when the cells were treated with DNA gyrase inhibitors, cspH mRNA was not induced upon nutrient up-shift. The low level of DNA superhelical density at the cspH promoter in part affected the expression of cspH mRNA in vitro. In addition, a fis-deficient strain had a lower level of cspH mRNA than the wild-type upon nutrient up-shift. Finally, a cspH-lacZ construct, in which the putative binding region for Fis was deleted in the cspH promoter, expressed a low level of LacZ, in contrast to the native cspH-lacZ construct.

References

Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K Mizuuchi, H A Nash
Feb 1, 1992·Molecular Microbiology·K Drlica
Dec 1, 1992·Journal of Bacteriology·C A BallR C Johnson
Nov 1, 1992·Molecular Microbiology·S E Finkel, R C Johnson
Nov 2, 1990·Cell·M B Schmid
Sep 1, 1987·Microbiological Reviews·K Drlica, J Rouviere-Yaniv
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·K NewkirkG T Montelione
Apr 1, 1995·Journal of Bacteriology·R OsunaR C Johnson
Jun 1, 1995·Journal of Bacteriology·P R JensenH V Westerhoff
Feb 16, 1996·Journal of Molecular Biology·K R BenjaminN R Cozzarelli
Apr 1, 1996·Molecular Microbiology·M van WorkumH V Westerhoff
Jan 3, 1997·The Journal of Biological Chemistry·W JiangM Inouye
Aug 1, 1997·Trends in Microbiology·Y C Tse-DinhR Menzel
Nov 13, 1998·Journal of Bacteriology·M B Beach, R Osuna
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·W BaeK Severinov
Mar 30, 2001·Biochimie·A TraversG Muskhelishvili
Apr 25, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K Yamanaka, M Inouye

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Citations

Jan 20, 2007·Molecular Microbiology·Dalit Weinstein-Fischer, Shoshy Altuvia
Jun 19, 2008·FEMS Microbiology Letters·Julia S SabirovaPeter N Golyshin

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