Expression of CYP1C1 and CYP1A in Fundulus heteroclitus during PAH-induced carcinogenesis.

Aquatic Toxicology
Lu WangK L Willett

Abstract

CYP1C1 is a relatively newly identified member of the cytochrome P450 family 1 in teleost fish. However, CYP1C1's expression and physiological roles relative to the more recognized CYP1A in polycyclic aromatic hydrocarbons (PAHs) induced toxicities are unclear. Fundulus heteroclitus fry were exposed at 6-8 days post-hatch (dph) and again at 13-15dph for 6h to dimethyl sulfoxide (DMSO) control, 5mg/L benzo[a]pyrene (BaP), or 5mg/L dimethylbenzanthracene (DMBA). Fry were euthanized at 0, 6, 18, 24 and 30h after the second exposure. In these groups, both CYP1A and CYP1C1 protein expression were induced within 6h after the second exposure. Immunohistochemistry (IHC) results from fry revealed strongest CYP1C1 expression in renal tubular and intestinal epithelial cells. Additional fish were examined for liver lesions 8 months after initial exposure. Gross lesions were observed in 20% of the BaP and 35% of the DMBA-treated fish livers. Histopathologic findings included foci of cellular alteration and neoplasms, including hepatocellular adenoma, hepatocellular carcinoma and cholangioma. Strong CYP1A immunostaining was detected diffusely in altered cell foci and on the invading margin of hepatocelluar carcinomas. Lower CYP1A expression ...Continue Reading

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Citations

Apr 2, 2013·Environmental Toxicology and Pharmacology·Xiefan FangKristine L Willett
Sep 12, 2015·Journal of Environmental Sciences (China)·Jin Wuk LeeJae-Seong Lee
Aug 2, 2015·Biochimica Et Biophysica Acta·John J StegemanPhilippe Urban
May 24, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Xiefan FangKristine L Willett
May 24, 2016·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·D R BrownR T Di Giulio
Dec 22, 2019·Extremophiles : Life Under Extreme Conditions·Ahmad Fareez Ahmad RosleeSiti Aqlima Ahmad
Jun 7, 2020·Biochemical Genetics·Shaolin XieJixing Zou
Jul 13, 2019·Ecotoxicology and Environmental Safety·Clei Endrigo PiazzaAfonso Celso Dias Bainy
Aug 2, 2021·Marine Environmental Research·Adélaïde LereboursJasmine Nahrgang

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