Expression of cystic fibrosis transmembrane conductance regulator alters the responses to hypotonic cell swelling and ATP of Chinese hamster ovary cells

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
I E ThieleR Greger

Abstract

The aim of this study was to examine whether the stable expression of wild-type cystic fibrosis transmembrane conductance regulator (CFTR) in Chinese hamster ovary (CHO) cells alters the properties of these cells towards hypotonic cell swelling and ATP. According to many previous studies this was not expected a priori, since overexpression of CFTR should not affect the conductive pathways upregulated by the purinergic agonist or cell swelling. Three types of CHO cells were examined: a control group of normal CHO cells; a group of CFTR-CHO cells stably expressing wild-type CFTR at high levels (CHO-CFTR), and a group delta F508-CFTR-CHO cells, stably expressing the frequent mutation delta F508 CFTR (CHO-delta F508). Whole cell patch-clamp studies were performed to measure the membrane voltage (Vm), the membrane conductance (Gm), and the membrane capacitance (C(m)). Hypotonic cell swelling (Hypo, 150 mosm/l) was used, because it activates Cl- and K+ channels and enables the cell to extrude KCl in many cells, and ATP because it is known to activate Ca(2+)-regulated channels in a large variety of cells. Hypo depolarized all three types of cells. This depolarization was accompanied by an increase in Cl- conductance. The selectivity o...Continue Reading

Citations

Dec 24, 2008·Journal of Neural Engineering·Han Bing WangRyan J Gilbert
Mar 18, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Astrid StumpfHermann Schillers
Dec 19, 2020·Frontiers in Cell and Developmental Biology·Raquel CenteioKarl Kunzelmann

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